| 基于16SrDNA测序法分析大肠息肉患者肠道菌群的特征 |
| 投稿时间：2024-09-30 修订日期：2024-12-11 点此下载全文 |
| 引用本文：付新年,马驰,王鑫鑫,马笑盈,罗江焰,郑盛,杨涓.基于16SrDNA测序法分析大肠息肉患者肠道菌群的特征[J].医学研究杂志,2025,54(5):93-98, 165 |
| DOI：
10.11969/j.issn.1673-548X.2025.05.018 |
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| 基金项目:云南省教育厅科学研究基金资助项目(2024Y920) |
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| 中文摘要:目的 分析大肠非腺瘤性息肉、普通腺瘤性息肉和进展期腺瘤性息肉患者肠道菌群变化及其差异。方法 收集2022年9月～2023年12月就诊于大理大学第二附属医院消化内科大肠息肉患者及同期健康体检者的粪便样本,通过16SrDNA基因测序法对肠道菌群测序数据进行分析。结果 Alpha多样性指数分析结果显示,非腺瘤组、普通腺瘤组和进展期腺瘤组菌群多样性及丰度较健康对照组均有所下降(P均＜0.05)。在门水平上,与健康组比较,非腺瘤组、普通腺瘤组和进展期腺瘤组变形菌门相对丰度升高,拟杆菌门相对丰度降低。进展期腺瘤组患者弯曲菌门相对丰度较非腺瘤组和普通腺瘤组显著低于健康对照组(P＝0.005)。在属水平上,非腺瘤组、普通腺瘤组和进展期腺瘤组患者肠道大肠杆菌-志贺菌属和链球菌属相对丰度高于健康对照组,普拉粪杆菌属和琼脂杆菌属相对丰度低于健康组(P＜0.05)。进展期腺瘤组患者肠道UBA1819菌属相对丰度高于非腺瘤组和普通腺瘤组(P＝0.038)。结论 大肠非腺瘤性息肉、普通腺瘤性息肉和进展期腺瘤性息肉患者肠道菌群多样性及丰度均下降,进展期腺瘤性息肉患者UBA1819菌属水平升高。 |
| 中文关键词:大肠息肉 肠道菌群 16SrDNA测序 进展期腺瘤性息肉 UBA1819菌属 |
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| Characteristics of Intestinal Flora in Patients with Large Intestine Polyps were Analyzed by 16SrDNA Sequencing. |
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| Abstract:Objective The changes and differences of intestinal flora in patients with non-adenomatous polyps,common adenomatous polyps and advanced adenomatous polyps were analyzed.Methods Stool samples of patients with large intestine polyps meeting the ataxic standard and healthy physical examination subjects were collected from September 2022 to December 2023 in the Department of Gastroenterology of the Second Affiliated Hospital of Dali University,and the intestinal flora sequencing data were analyzed by 16SrDNA gene sequencing method.Results The results of Alpha diversity index showed that the diversity and abundance of bacteria in non-adenoma group,common adenoma group and advanced adenoma group were decreased compared with healthy control group (P＜0.05).At the phylum level, compared with the healthy group,the relative abundance of Proteobacteria increased in the non-adenoma group,the common adenoma group and the advanced adenoma group, while the relative abundance of Bacteroidetes decreased.The relative abundance of campylobacter in advanced adenoma group was significantly lower than that in non-adenoma group and common adenoma group (P＝0.005).At the genus level,the relative abundances of Escherichia coli Shigella and Streptococcus in the non-adenoma group,common adenoma group and advanced adenoma group were higher than those in the healthy control group,while the relative abundances of Fecalis praecalis and Agathobacter were lower than those in the healthy group (P＜0.05).The relative abundance of UBA1819 bacteria in the advanced adenoma group was higher than that in the non-adenoma group and the common adenoma group (P＝0.038).Conclusion Intestinal flora diversity and abundance decreased in patients with non-adenomatous polyps,common adenomatous polyps and advanced adenomatous polyps,while UBA1819 bacteria level increased in patients with advanced adenomatous polyps. |
| keywords:large intestine polyp Intestinal flora 16SrDNA sequencing Advanced adenomatous polyps UBA1819 |
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