| 结直肠癌中TMED4与免疫微环境的关系 |
| 投稿时间：2024-12-25 修订日期：2025-02-21 点此下载全文 |
| 引用本文：周荡,李俊蓉,刘琦,赵寒冰.结直肠癌中TMED4与免疫微环境的关系[J].医学研究杂志,2025,54(6):127-132 |
| DOI：
10.11969/j.issn.1673-548X.2025.06.023 |
| 摘要点击次数: 14 |
| 全文下载次数: 8 |
|
| 基金项目:贵州省卫生健康委科学技术基金资助项目(gzwkj2022-050)；贵州医科大学高层次人才启动基金项目{校博合J字[2020]071} |
|
| 中文摘要:目的 探讨TMED4在结直肠癌中表达的临床意义及其与免疫微环境的关系。方法 根据癌症基因组图谱(the cancer genome atlas,TCGA)数据库以R语言分析TMED4在结直肠癌中的表达水平及其预后价值,收集临床样本进行聚合酶链反应(polymerase chain reaction,PCR)验证TMED4在结直肠癌中的表达情况,分析TMED4与临床病理特征的相关性。通过基因集富集分析(gene set enrichment analysis,GSEA)和免疫浸润分析检测TMED4的功能作用。结果 结直肠癌样本的TMED4表达水平上调。TMED4高表达与结直肠癌的病理分期、淋巴结转移和远距离转移密切相关。TMED4高表达与患者总生存期(overall survival,OS)及疾病特异性生存期(disease-specific survival,DSS)密切相关；单因素分析和多因素COX回归分析结果显示,TMED4是结直肠癌患者DSS的独立危险因素。受试者工作特征(receiver operating characteristic,ROC)曲线分析显示TMED4具备成为结直肠癌诊断潜在标志物的可能性。GSEA揭示TMED4可能参与多种途径,主要集中于运输与分解代谢通路、聚糖的生物合成与代谢通路、脂代谢通路。结论 TMED4高表达与结直肠癌预后不良及免疫细胞浸润密切相关,可能作为判断结直肠癌预后的独立生物学标志物和潜在的治疗靶点。 |
| 中文关键词:结肠癌 TMED4 肿瘤标志物 免疫微环境 |
| |
| Association between TMED4 and the Immune Microenvironment in Colorectal Cancer. |
|
|
| Abstract:Objective To explore the clinical significance of TMED4 expression in colorectal cancer and its relationship with the immune microenvironment. Methods Based on the cancer genome atlas (TCGA) database, the expression level of TMED4 in colorectal cancer and its prognostic value were analyzed using R language. Clinical samples were collected for polymerase chain reaction (PCR) experiments to verify its expression, and the correlation with clinicopathological features and prognostic value were analyzed. The functional role of TMED4 was detected through gene set enrichment analysis (GSEA) and immune infiltration analysis. Results The expression level of TMED4 was upregulated in colorectal cancer samples. The expression level of TMED4 was closely associated with the pathological stage, lymphatic metastasis, and distant metastasis of colorectal cancer. The increase in TMED4 expression was closely related to the overall survival (OS) and disease-specific survival (DSS) of patients. Univariate analysis and multivariate COX analysis found that TMED4might be an independent high-risk factor for DSS in colorectal cancer patients. Receiver operating characteristic (ROC) curve analysis showed that TMED4has the potential to become a potential biomarker for the diagnosis of colorectal cancer. GSEA revealed that TMED4might be involved in multiple pathways, mainly concentrated in transport and catabolic pathways, glycan biosynthesis and metabolism pathways, and lipid metabolism pathways. Conclusion High expression of TMED4 is closely related to poor prognosis of colorectal cancer and immune cell infiltration. It may serve as an independent biomarker for predicting the prognosis of colorectal cancer and a potential therapeutic target. |
| keywords:Colorectal cancer TMED4 Tumor marker Immune microenvironment |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
