| 颅骨撞击构建小鼠视皮层TBI模型及视功能评估 |
| 投稿时间：2025-02-22 修订日期：2025-07-30 点此下载全文 |
| 引用本文：王兆龙,蓝志达,崔迪娜,赵乐文,方伟群.颅骨撞击构建小鼠视皮层TBI模型及视功能评估[J].医学研究杂志,2025,54(8):42-47 |
| DOI：
10.11969/j.issn.1673-548X.2025.08.008 |
| 摘要点击次数: 15 |
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| 基金项目:国家自然科学基金资助项目(32271023)；上海市浦江人才计划项目(22PJ1408900) |
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| 中文摘要:目的 建立一种创伤性脑损伤(traumatic brain injury, TBI)的小鼠模型,以探讨视皮层损伤对视皮层神经生物学及认知功能的影响。方法 采用立体定位技术,对8周龄C57BL/6小鼠的初级视皮层(primary visual cortex, V1)上方颅骨进行局部不同强度撞击(撞击速度1~4m/s),同时避免造成硬脑膜破损。撞击2周后,通过免疫荧光染色及共聚焦显微成像分析神经元数量变化,利用相似物体识别和2h新物体识别实验评估模型小鼠视觉功能,利用旷场实验和24h新物体识别实验评估模型小鼠运动、焦虑和认知等功能。结果 当撞击速度为4m/s时,小鼠V1浅层神经元数量降低约10%(P<0.01),与轻微脑损伤患者相当。在相似物体识别实验中,TBI模型小鼠的辨别指数(recognition index, RI)相比对照组显著降低(0.51±0.06 vs 0.58±0.05,P<0.05)；在2h新物体识别实验中,TBI模型小鼠的RI与对照组比较,差异无统计学意义(P>0.05)；在旷场实验中,TBI模型小鼠的运动速度、中央区停留时间相比对照组差异无统计学意义(P>0.05)；在24h新物体识别实验中,TBI模型小鼠的RI与对照组差异无统计学意义(P>0.05)。结论 以物理撞击方式(速度4m/s、深度0.5mm、停留时间0.5s)建立V1浅层损伤的小鼠模型,其视觉功能受到轻微损伤,并且不影响其运动及记忆功能,为研究TBI后视觉障碍的恢复机制提供了新的研究工具。 |
| 中文关键词:视皮层 皮层损伤 TBI 神经元 |
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| Establishment of a Traumatic Brain Injury (TBI) Model in Mouse Visual Cortex via Skull Impact and Assessment of Visual Function. |
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| Abstract:Objective To establish a mouse model of traumatic brain injury (TBI), to investigate the effects of visual cortex injury on neurobiology and cognitive function of the visual cortex.Methods Stereotaxic-guided graded cortical impacts (impact velocity was 1-4m/s) were administered to the primary visual cortex (V1)-associated skull surface in C57BL/6mice (8-week-old) with intact dura mater. Two weeks after the impact, the changes of neuronal numbers were analyzed by immunofluorescence staining and confocal microscope imaging. Similar and 2-hour novel object recognition tests were used to evaluate the model visual function of mice. Open field tests and 24-hour novel object identification tests were used to assess the model non-visual abilities of mice, such as mobility, anxiety, and cognition. Results The number of superficial neurons in the mice′s V1dropped by roughly 10% (P<0.01) with an impact velocity of 4m/s, which was similar to what happens in individuals with mild brain injury. The recognition index (RI) of the TBI model mice was found to be significantly lower than that of the control group in the similar object recognition test (0.51±0.06 vs 0.58±0.05, P<0.05). However, in the 2-hour novel object recognition test, no statistically significant difference in RI was detected between the TBI model mice and the control group (P>0.05). Furthermore, the open field test indicated no significant disparities in locomotion speed or the time spent in the central area between the TBI model mice and the control group (P>0.05). Similarly, the 24-hour novel object recognition test revealed no significant difference in RI between the TBI model mice and the control group (P>0.05). Conclusion A mouse model simulating superficial injury to V1 was created through a controlled physical impact, characterized by a velocity of 4m/s, a penetration depth of 0.5mm, and a dwell time of 0.5seconds. The visual capabilities of the model mice exhibited mild impairment, whereas their motor and cognitive functions remained intact. This model offers a novel research tool for exploring the mechanisms underlying the recovery of visual deficits following TBI. |
| keywords:Visual cortex Cortical injury TBI Neuron |
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