| lncRNA OIP5-AS1和miR-22-3p在胃癌组织中的表达及其与临床病理特征和预后的关系 |
| 投稿时间：2024-12-26 修订日期：2025-03-10 点此下载全文 |
| 引用本文：王巍松,倪银海,钱蕾.lncRNA OIP5-AS1和miR-22-3p在胃癌组织中的表达及其与临床病理特征和预后的关系[J].医学研究杂志,2025,54(8):88-93, 107 |
| DOI：
10.11969/j.issn.1673-548X.2025.08.015 |
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| 基金项目:浙江省医药卫生科技计划项目(2020KY993) |
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| 中文摘要:目的 探究长链非编码核糖核酸Opa相互作用蛋白5反义转录本1(long non-coding ribonucleic acid OPA-interacting protein 5 antisense transcript 1,lncRNA OIP5-AS1)和微小核糖核酸-22-3p(microribonucleic acid 22-3p,miR-22-3p)在胃癌组织中的表达及其与临床病理特征和预后的关系。方法 回顾性选取2017年6月~2020年11月在诸暨市中心医院确诊为胃癌的患者106例为研究对象。术中收集胃癌患者癌组织和癌旁组织(距病灶＞5cm),分别设为胃癌组和癌旁组。定量反转录聚合酶链反应(qRT-PCR)用于检测各组织中lncRNA OIP5-AS1和miR-22-3p水平。本研究中分为预后不良组(死亡)40例和预后良好组66例。Pearson法分析胃癌患者癌组织lncRNA OIP5-AS1和miR-22-3p水平的关系；Kaplan-Meier生存分析评估lncRNA OIP5-AS1和miR-22-3p表达水平与患者预后的关系。COX回归分析预后影响因素。结果 与癌旁组比较,胃癌组lncRNA OIP5-AS1水平显著升高,miR-22-3p水平显著降低(P均＜0.05)。荧光素酶活性检测显示lncRNA OIP5-AS1和miR-22-3p存在靶向关系。癌组织中lncRNA OIP5-AS1和miR-22-3p表达水平呈负相关(rp=-0.426,P＜0.001)。lncRNA OIP5-AS1和miR-22-3p表达水平与肿瘤分期、分化程度密切相关(P均＜0.05)。与预后良好组比较,预后不良组患者癌组织中lncRNA OIP5-AS1水平显著升高,miR-22-3p水平显著降低(P均＜0.05)。Kaplan-Meier分析结果显示,lncRNA OIP5-AS1高表达组患者3年生存率显著低于低表达组(χ2=27.667,P＜0.001)；miR-22-3p高表达组患者3年生存率显著高于低表达组(χ2=19.876,P＜0.001)。COX回归分析结果显示,lncRNA OIP5-AS1(HR=3.652)和miR-22-3p(HR=0.748)表达均为胃癌患者预后的影响因素(P均＜0.05)。结论 胃癌患者癌组织中lncRNA OIP5-AS1呈高表达,miR-22-3p呈低表达,两者存在负相关关系,且其表达水平均与胃癌的临床病理特征和患者预后密切相关。 |
| 中文关键词:长链非编码核糖核酸Opa相互作用蛋白5反义转录本1 微小核糖核酸-22-3p 胃癌 临床病理特征 预后 |
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| Expressions of LncRNA OIP5-AS1 and miR-22-3p in Gastric Cancer Tissue and Their Relationships with Clinicopathological Features and Prognosis. |
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| Abstract:Objective To investigate the expressions of long non-coding ribonucleic acid OPA-interacting protein 5 antisense transcript 1 (lncRNA OIP5-AS1) and microribonucleic acid 22-3p (miR-22-3p) in gastric cancer tissue and their relationships with clinicopathological features and prognosis. Methods A total of 106 patients diagnosed with gastric cancer in Zhuji Central Hospital from June 2017 to November 2020 were retrospectively enrolled as the study subjects. Cancer tissue and adjacent tissue (>5cm from the lesion) were collected during surgery and assigned into the gastric cancer group and adjacent group, respectively. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was applied to detect the levels of lncRNA OIP5-AS1 and miR-22-3p in various tissues. The prognoses were poor (death) in 40 patients (poor prognosis group) and good in 66 patients (good prognosis group). Pearson method was applied to analyze the relationship between lncRNA OIP5-AS1 and miR-22-3p levels in cancer tissues. Kaplan-Meier method was applied to analyze the relationships of the expression levels of lncRNA OIP5-AS1 and miR-22-3p with prognosis. COX regression analysis was performed to analyze the factors influencing the prognosis. Results Compared with the adjacent group, the gastric cancer group had significantly increased lncRNA OIP5-AS1 level and significantly reduced miR-22-3p level (all P<0.05). Luciferase assay showed that lncRNA OIP5-AS1had a targeting relationship with miR-22-3p. The expression level of lncRNA OIP5-AS1 was negatively correlated with that of miR-22-3p in cancer tissues (rp=-0.426, P<0.001). The expression levels of lncRNA OIP5-AS1 and miR-22-3p were closely related to tumor staging and differentiation (all P<0.05). Compared with the good prognosis group, the poor prognosis group had significantly increased lncRNA OIP5-AS1 level and significantly reduced miR-22-3p level in cancer tissues (all P<0.05). Kaplan-Meier method reveled that the 3-year survival rate was significantly lower in the high lncRNA OIP5-AS1 expression group than in the low lncRNA OIP5-AS1 expression group (χ2=27.667, P<0.001) but was significantly higher in the high miR-22-3p expression group than in the low miR-22-3p expression group (χ2=19.876, P<0.001). COX regression showed that the expressions of lncRNA OIP5-AS1 (HR=3.652) and miR-22-3p (HR=0.748) were the prognostic factors for gastric cancer patients (all P<0.05). Conclusion LncRNA OIP5-AS1 is highly expressed in gstric cancer tissues, while miR-22-3p is lowly expressed. The two are negatively correlated, and both of them are closely related to the clinicopathological features and prognosis of gastric cancer. |
| keywords:Long chain non coding ribonucleic acid Opa interacting protein 5 antisense transcript 1 Microribonucleic acid-22-3p Gastric cancer Clinical pathological features Prognosis |
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