| 热敏灸干预对变应性鼻炎大鼠PD-1及PI3K/Akt/mTOR信号通路相关蛋白表达的影响 |
| 投稿时间：2025-03-17 修订日期：2025-04-09 点此下载全文 |
| 引用本文：林萌,熊俊,李雨芯,况迎萍,姚丽君,胡菡.热敏灸干预对变应性鼻炎大鼠PD-1及PI3K/Akt/mTOR信号通路相关蛋白表达的影响[J].医学研究杂志,2025,54(9):62-70 |
| DOI：
10.11969/j.issn.1673-548X.2025.09.012 |
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| 基金项目:国家自然科学基金资助项目(82060893)；江西省博士后科研项目择优资助项目(2020KY08)；江西省博士后日常经费资助项目(2021RC13)；江西省教育厅科技计划重点项目(GJJ190629) |
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| 中文摘要:目的 探讨热敏灸干预对卵清蛋白(ovalbumin, OVA)诱导的变应性鼻炎(allergic rhinitis, AR)大鼠程序性死亡受体-1(programmed death receptor-1, PD-1)及其下游磷脂酰肌醇-3激酶/蛋白激酶/哺乳动物雷帕霉素靶蛋白(PI3K/Akt/mTOR)信号通路相关蛋白表达的影响,阐明其治疗AR的潜在机制。方法 将60只SD大鼠随机分为空白组(n=10)和造模组(n=50),分别以0.9%氯化钠注射液及OVA制备AR模型。造模成功后,将造模大鼠随机分为模型组(n=10)、艾灸组(n=20)、阳性药组(n=10)及PD-1抑制剂组(n=10)。空白组不干预；模型组和阳性药组分别予以0.9%氯化钠注射液/丙酸氟替卡松鼻喷雾剂滴鼻,8微升/侧,每天1次,共21天；PD-1抑制剂组于敏化激发前阻断干预7天；艾灸组以热敏灸艾条悬灸肺俞穴(每天1次,每次40min),记录干预过程中的体、尾温变化,共21天,末次治疗后按照体、尾温变化情况将艾灸组划分为艾灸组(n=9)和热敏灸组(n=11)。干预后,采用苏木精-伊红染色观察鼻黏膜形态,免疫荧光法与Western blot法检测PD-1及PI3K/Akt/mTOR信号通路相关蛋白表达,实时荧光定量聚合酶链反应(real-time fluorescence quantitative polymerase chain reaction, RT-qPCR)法检测脾脏组织相关基因表达。结果 与空白组比较,模型组大鼠PD-1及PI3K、Akt、mTOR表达量及其磷酸化水平均显著升高(P＜0.05)。经热敏灸干预后,PD-1及PI3K、Akt、mTOR的蛋白和mRNA表达,以及p-PI3K/PI3K、p-Akt/Akt、p-mTOR/mTOR均较模型组显著下降(P＜0.05),与艾灸组、阳性药组及PD-1抑制剂组具有相似的下调趋势。结论 热敏灸“肺俞穴”可有效改善AR大鼠鼻部炎症及组织损伤,其机制可能与下调PD-1表达并抑制下游PI3K/Akt/mTOR信号通路活化有关。 |
| 中文关键词:变应性鼻炎 PI3K/Akt/mTOR PD-1 免疫调控 |
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| Effects of Heat-sensitive Moxibustion Intervention on the Expression of PD-1 and PI3K/Akt/mTOR Signaling Pathway-related Proteins in Rats with Allergic Rhinitis |
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| Abstract:Objective To explore the effects of heat-sensitive moxibustion intervention on the expression of programmed death receptor-1 (PD-1) and its downstream PI3K/Akt/mTOR signaling pathway-related proteins in ovalbumin (OVA)-induced allergic rhinitis (AR) rats, and to elucidate the potential mechanism of its therapeutic treatment of AR. Methods Sixty SD rats were randomly divided into the blank group (n=10) and the modeling group (n=50), and AR models were prepared with normal saline and OVA, respectively. After successful modeling, the modeled rats were randomly divided into model group (n=10), moxibustion group (n=20), positive drug group (n=10) and PD-1 inhibitor group (n=10). The blank group had no intervention; the model group and the positive drug group were given normal saline/fluticasone propionate nasal spray nasal drops, 8μl/side, once a day for 21days; the PD-1 inhibitor group was blocked from the intervention for 7days before sensitization was stimulated; the moxibustion group was treated with thermal moxibustion moxa strips suspended on the “feishu point” (once a day, 40minutes each time), and the changes of the body and tail temperatures were recorded in the course of the intervention, for a total of 21days. After the last treatment, the moxibustion group was divided into moxibustion group (n=9) and thermal moxibustion group (n=11) according to the changes in body and tail temperature. After the intervention, hematoxylin-eosin staining was used to observe the morphology of nasal mucosa, immunofluorescence and Western blot was used to detect the expressions of PD-1 and PI3K/Akt/mTOR-related proteins, and real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of related genes in spleen tissues. Results Compared with the blank group, the expression of PD-1 and PI3K, Akt, mTOR and their phosphorylation levels were significantly elevated in the rats of model group (P<0.05). After the intervention of thermal moxibustion, the protein and mRNA expression of PD-1 and PI3K, Akt, mTOR, as well as the ratios of p-PI3K/PI3K, p-Akt/Akt, and p-mTOR/mTOR were significantly decreased compared with that of the model group (P<0.05), and the down-regulation tendency was similar to that of the moxibustion group, the positive drug group and the PD-1 inhibitor group. Conclusion Thermal moxibustion at the “feishu point” can effectively improve nasal inflammation and tissue damage in AR rats, and its mechanism may be related to the down-regulation of PD-1 expression and the inhibition of downstream PI3K/Akt/mTOR signaling pathway activation. |
| keywords:Allergic rhinitis PI3K/Akt/mTOR PD-1 Immunomodulation |
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