| 基于网络药理学研究连芩解毒方的免疫调节机制 |
| 投稿时间：2025-04-08 修订日期：2025-04-12 点此下载全文 |
| 引用本文：龙远雄,韩蜜.基于网络药理学研究连芩解毒方的免疫调节机制[J].医学研究杂志,2025,54(9):91-97 |
| DOI：
10.11969/j.issn.1673-548X.2025.09.016 |
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| 基金项目:湖南省自然科学基金项目医卫行业联合基金项目(2025JJ80970)；湖南省卫生健康委员会科研计划项目(D202302048885);湖南中医药大学中药粉体与创新药物省部共建国家重点实验室培育基地开放基金项目(24PTKF1022) |
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| 中文摘要:目的 基于网络药理学方法研究连芩解毒方的免疫调节作用机制。方法 通过中药系统药理学数据库与分析平台(Troditional Chinese Medicine Systems Pharmacology Database and Analysis Platform, TCMSP)和GeneCards数据库,分别获取连芩解毒方的活性成分及其潜在靶点、免疫调节相关靶点,取交集靶点进行分析。构建蛋白互作(protein-protein interaction,PPI)网络和化合物-靶点网络,筛选关键活性成分和核心作用靶点。采用分子对接技术验证关键活性成分与核心靶点的结合活性,并利用DAVID(The Database for Annotation,Visualization, and Integrated Discovery)数据库对交集靶点进行基因本体论(Gene Ontology, GO)功能及京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes, KEGG)通路富集分析。结果 连芩解毒方共有143个成分和164个靶点,其中有130个成分、79个靶点与免疫调节有关,占比分别为90.91%和48.17%；其中关键化合物13个,核心靶基因18个,Degree值排名前4位的核心靶点与排名前3位的关键化合物体现较好的结合性。连芩解毒方参与免疫调节主要影响272个生物学过程,58条通路。结论 连芩解毒方可能通过槲皮素、山奈酚、β-谷甾醇等关键化合物作用于肿瘤坏死因子(tumor necrosis factor, TNF)、白细胞介素-6 (interleukin-6, IL-6)等核心靶点,影响蛋白质磷酸化、细胞质、细胞核等生物学过程,调节代谢途径等关键通路来发挥其免疫调节作用,本研究为后续免疫性疾病的治疗提供了参考依据。 |
| 中文关键词:网络药理学 分子对接 连芩解毒方 免疫调节 |
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| Immunoregulation Mechanism of Lianqin Jiedu Formula based on Network Pharmacology |
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| Abstract:Objective To study the immunomodulatory mechanism of lianqin jiedu formula based on network pharmacology methods. Methods Using Troditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) and GeneCards databases, the active ingredients and potential targets of lianqin jiedu formula was obtained, as well as immune regulation related targets, and the intersection targets was analyzed. The protein-protein interaction (PPI) network and compound target network was constructed, key active ingredients and core action targets were screened. Molecular docking technology was used to verify the binding activity between key active components and core targets, and the intersection targets were analyzed for Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment using the The Database for Annotation,Visualization, and Integrated Discovery (DAVID) database.Results The Lianqin jiedu formula consists of 143 components and 164 targets, of which 130 components and 79 targets were related to immunoregulation, accounting for 90.91% and 48.17% respectively; among them, there were 13 key compounds and 18 core target genes. The top 4 core targets with the highest degree values showed good binding affinity with the top 3 key compounds. The lianqin jiedu formula was involved in immunoregulation and mainly affects 272 biological processes and 58 pathways. ConclusionThe lianqin jiedu formula may exert its immunoregulation effects by targeting core targets such as tumor necrosis factor (TNF) and interleukin-6 (IL-6) through key compounds such as quercetin, kaempferol, and β- sitosterol, affecting biological processes such as protein phosphorylation, cytoplasm, and nucleus, and regulating key pathways such as metabolic pathways. This study provides a reference for the treatment of subsequent immune diseases. |
| keywords:Network pharmacology Molecular docking Lianqin jiedu formula Immunoregulation |
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