| Nrf2/Keap1/p62通路在胸外科术后肺部炎症患者体内的价值 |
| 投稿时间：2025-03-19 修订日期：2025-04-25 点此下载全文 |
| 引用本文：卜玲玲,李亮,段雨情,丁萌,刘磊,童小慧,韩荣春,樊志伟.Nrf2/Keap1/p62通路在胸外科术后肺部炎症患者体内的价值[J].医学研究杂志,2025,54(9):123-127 |
| DOI：
10.11969/j.issn.1673-548X.2025.09.021 |
| 摘要点击次数: 26 |
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| 基金项目:安徽省自然科学基金资助项目(2208085QH271)；阜阳市卫生健康科研项目(202349) |
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| 中文摘要:目的 观察Nrf2/Keap1/p62信号通路相关分子在胸外科术后肺部炎症患者体内的动态变化,并探讨其与预后的关系。方法 收集2023年6月～2024年6月安徽中医药大学附属太和中医院胸外科收治的胸外科术后相关肺炎患者60例为研究对象,采集患者术前、术后第1天、术后第3天和术后第7天的静脉血,检测外周血中超氧化物歧化酶(superoxide dismutase,SOD)、一氧化氮(nitric oxide,NO)、丙二醛(malondialdehyde, MDA)、活性氧(reactive oxygen species,ROS)的水平以及Nrf2、Keap1、p62mRNA的表达,分析各个指标与胸外科术后并发肺部感染预后的相关性。结果 患者术后SOD比术前降低,术后第1天达到最低值。MDA、ROS在术后出现了上升,但在术后第7天缓慢下降。NO水平对比术前无明显变化。与手术前比较,患者手术后Nrf2和Keap1的表达水平在术后第1天出现了明显下降,随后缓慢上升；p62表达水平在术后第1天上升到最高峰,随后下降。肺功能指标的测定结果显示,患者手术后发生了肺部感染,肺功能水平比手术前出现了明显的下降。经受试者工作特征曲线图分析,SOD、ROS、Nrf2、Keap1、p62在胸外科术后肺部炎症患者体内的动态变化预测预后的价值良好。结论 胸外科术后肺部炎症患者体内 Nrf2/Keap1/p62通路相关因子变化与胸外科术后肺部炎症患者预后相关,可从相关基因表达水平变化的角度有效预测患者的预后情况。 |
| 中文关键词:Nrf2/Keap1/p62通路 胸外科术后 肺部炎症 预后 |
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| Value of the Nrf2/Keap1/p62 Pathway in Patients with Postoperative Pulmonary Inflammation after Thoracic Surgery |
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| Abstract:Objective To observe the dynamic changes of molecules related to the Nrf2/Keap1/p62 signaling pathway in patients with postoperative pulmonary inflammation after thoracic surgery, and explore its relationship with prognosis. Methods Sixty patients with postoperative pneumonia after thoracic surgery who met the criteria in the Department of Thoracic Surgery of Taihe County Hospital of Traditional Chinese Medicine from June 2023 to June 2024 were selected as the research subjects. Venous blood was collected from the patients on the day before surgery, the first day after surgery, the third day after surgery, and the seventh day after surgery to detect the levels of superoxide dismutase (SOD), nitric oxide (NO), malondialdehyde (MDA), reactive oxygen species (ROS) in peripheral blood, as well as the expression of Nrf2, Keap1, and p62 mRNA. The correlations between these indicators and the prognosis of postoperative pulmonary infection after thoracic surgery were analyzed. Results The SOD level of the patients decreased after the operation compared with that before the operation, reaching the lowest value on the first day after the operation. The levels of MDA and ROS increased after the operation, but slowly decreased on the seventh day after the operation. There was no significant change in the NO level compared with that before the operation. Compared with before the operation, the expression levels of Nrf2 and Keap1 in the patients decreased significantly on the first day after the operation, and then slowly increased. The expression level of p62 increased to the peak on the first day after the operation, and then decreased. The results of lung function index measurement showed that the patients had pulmonary infection after the operation, and the lung function level decreased significantly compared with that before the operation. Through ROC curve analysis, the dynamic changes of SOD, ROS, Nrf2, Keap1 and p62 in the patients with pulmonary inflammation after thoracic surgery have good predictive value for prognosis. Conclusion The changes of Nrf2/Keap1/p62 signaling pathway-related factors in patients with post-thoracic surgery-induced pulmonary inflammation are significantly correlated with the prognosis of these patients. Such changes can effectively predict the prognosis of patients from the perspective of the expression levels of related genes. |
| keywords:Nrf2/Keap1/p62 pathway Postoperative thoracic surgery Pulmonary inflammation Prognosis |
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