| PDIA3在子宫内膜癌中的表达水平及对Ishikawa细胞的影响 |
| 投稿时间：2025-03-02 修订日期：2025-04-11 点此下载全文 |
| 引用本文：李梦璇,侯倩玉,辛聪,经莉.PDIA3在子宫内膜癌中的表达水平及对Ishikawa细胞的影响[J].医学研究杂志,2025,54(9):147-153 |
| DOI：
10.11969/j.issn.1673-548X.2025.09.025 |
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| 中文摘要:目的 探究蛋白质二硫键异构酶A3(protein disulfide isomerase A3, PDIA3)在子宫内膜癌(endometrial cancer,EC)组织中的表达水平、临床意义及其对Ishikawa细胞的影响。方法 利用TCGA数据库分析不同内膜组织中PDIA3的表达水平差异,探究其与EC患者临床病理参数及预后间的联系；利用免疫组化法(immunohistochemical,IHC)检测临床内膜组织中PDIA3的表达水平,分析不同表达水平EC患者的临床病理参数、预后及影响其预后的危险因素；利用Transwell实验、划痕实验、CCK-8实验检测敲低PDIA3表达的Ishikawa细胞的增殖、侵袭和迁移能力。结果 TCGA数据库及临床标本中EC组织PDIA3表达水平高于正常内膜组织(P＜0.05),TCGA数据库中EC组织PDIA3表达水平G3级组高于G1及G2级组(P＜0.05),临床标本中EC组织PDIA3表达水平国际妇产科联盟(Federation International of Gynecology and Obstetrics, FIGO)分期Ⅱ~Ⅳ期组高于Ⅰ期组、有淋巴结转移组高于无淋巴结转移组、高分化组高于低分化组(P＜0.05)；COX回归分析结果显示,FIGO分期、PDIA3表达水平是影响EC患者预后的独立危险因素；TCGA数据库中EC患者PDIA3高表达组生存时间短于低表达组(P＜0.05),临床患者PDIA3阳性组生存率低于阴性组(P＜0.05)；Transwell实验、划痕实验及CCK-8实验显示PDIA3抑制组细胞的侵袭和迁移能力较正常组及阴性对照组显著降低(P<0.05)。结论 PDIA3在EC组织中呈高表达,与肿瘤恶性程度和患者不良预后相关；靶向抑制PDIA3的表达可降低Ishikawa细胞的增殖、侵袭和迁移能力。 |
| 中文关键词:子宫内膜癌 PDIA3 Ishikawa细胞 TCGA数据库 |
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| Expression Level of PDIA3 in Endometrial Cancer and Its Impact on Ishikawa Cells |
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| Abstract:Objective To explore the expression level, clinical significance of protein disulfide isomerase A3 (PDIA3) in endometrial cancer (EC) tissues, and its effects on Ishikawa cells. Methods Using the TCGA database to analyze the differential expression levels of PDIA3 in different endometrial tissues and explore its relationship with clinical pathological parameters and prognosis of EC patients; Using immunohistochemistry (IHC) to detect the expression level of PDIA3 in clinical endometrial tissue, analyze the clinical pathological parameters, prognosis, and risk factors affecting the prognosis of EC patients with different expression levels; Using Transwell assay, scratch assay, and CCK-8 assay to detect the proliferation, invasion, and migration ability of Ishikawa cells with knockdown PDIA3 expression. Results The expression level of PDIA3 in EC tissue was higher than that in normal endometrial tissue in the TCGA database and clinical specimens (P<0.05). The expression level of PDIA3 in EC tissue in the G3 grade group was higher than that in the G1 and G2 grade groups in the TCGA database (P<0.05). The expression level of PDIA3 in EC tissue in FIGO stage Ⅱ-Ⅳ group was higher than that in the stage Ⅰ group, the group with lymph node metastasis was higher than that without lymph node metastasis, and the highly differentiated group was higher than the poorly differentiated group in clinical specimens (P<0.05); COX regression analysis showed that Federation International of Gynecology and Obstetrics (FIGO) staging and PDIA3 expression level were the independent risk factors of prognosis in EC patients; the survival time of EC patients with high expression of PDIA3 in the TCGA database was shorter than that of the low expression group (P<0.05), and the survival rate of clinical patients with positive PDIA3 was shorter than that of the negative group (P<0.05); Transwell assay, scratch assay, and CCK-8 assay showed that the invasion and migration ability of cells in the PDIA3 inhibition group was significantly reduced compared to the normal group and negative control group (P<0.05). ConclusionPDIA3 is highly expressed in EC tissues and is associated with the malignancy of tumors and poor prognosis of patients; targeted inhibition of PDIA3 expression can reduce the proliferation, invasion, and migration ability of Ishikawa cells. |
| keywords:Endometrial cancer PDIA3 Ishikawa cells TCGA database |
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