| 人参皂苷Rh4抑制人骨肉瘤MG-63细胞增殖的药理学机制 |
| 投稿时间：2025-06-28 修订日期：2025-07-18 点此下载全文 |
| 引用本文：宗亿洲,刘典珅,袁长深,段戡,梅其杰,曾超,李彦宏,王栋梁,戴易.人参皂苷Rh4抑制人骨肉瘤MG-63细胞增殖的药理学机制[J].医学研究杂志,2025,54(12):37-43 |
| DOI：
10.11969/j.issn.1673-548X.2025.12.008 |
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| 基金项目:国家自然科学基金资助项目(82160912,82060875) |
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| 中文摘要:目的 探讨人参皂苷Rh4抗骨肉瘤的药效及其药理学机制。方法 首先,采用CCK-8实验、克隆形成实验、EdU法、流式细胞术验证人参皂苷Rh4抑制人骨肉瘤MG-63细胞增殖的药效；接着,通过网络药理学、生物信息学、靶点富集分析等方法,预测人参皂苷Rh4抗骨肉瘤的生物学机制及关键信号通路；最后,使用Western blot法验证预测得到的结果。结果 体外实验证实,人参皂苷Rh4能够有效抑制人骨肉瘤MG-63细胞增殖；网络药理学等预测结果表明,人参皂苷Rh4通过靶向ESR1、Akt1等靶点诱导人骨肉瘤MG-63细胞凋亡；靶点富集分析及Western blot法检测结果表明,人参皂苷Rh4通过磷酸肌醇3-激酶/蛋白激酶B(phosphoinositol 3-kinase/protein kinase B,PI3K/Akt)信号通路诱导人骨肉瘤MG-63细胞发生凋亡。结论 人参皂苷Rh4以核心基因ESR1和Akt1为靶点,通过PI3K/Akt信号通路诱导人骨肉瘤MG-63细胞凋亡,进而抑制细胞增殖。 |
| 中文关键词:网络药理学 生物信息学 体外验证 人参皂苷Rh4 骨肉瘤 |
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| Pharmacological Mechanism of Ginsenoside Rh4 Inhibiting the Proliferation of Human Osteosarcoma MG-63 Cells. |
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| Abstract:Objective To investigate the anti-osteosarcoma effect of ginsenoside Rh4 and its pharmacological mechanism. Methods Firstly, CCK-8 assay, clone formation assay, EdU assay and flow cytometry were used to verify the inhibitory effect of ginsenoside Rh4 on the proliferation of human osteosarcoma MG-63 cells. Then, network pharmacology, bioinformatics and target enrichment analysis were used to predict the biological mechanism and key signaling pathways of ginsenoside Rh4 against osteosarcoma. Finally, Western blot method was used to verify the predicted results. Results Ginsenoside Rh4 can effectively inhibit the proliferation of human osteosarcoma MG-63 cells in vitro. The results of network pharmacology show that ginsenoside Rh4may induce apoptosis of human osteosarcoma MG-63 cells by targeting ESR1 and Akt1. The results of target enrichment analysis and Western blot experiments showed that ginsenoside Rh4 induced the apoptosis of human osteosarcoma MG-63 cells through phosphoinositol 3-kinase/protein kinase B (PI3K/Akt) signaling pathway. Conclusion Ginsenoside Rh4 targets the core genes ESR1 and Akt1 to induce apoptosis of human osteosarcoma MG-63 cells through the PI3K/Akt signaling pathway, and thereby inhibits cell proliferation. |
| keywords:Network pharmacology Bioinformatics In vitro validation Ginsenoside Rh4 Osteosarcoma |
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