| 老年小鼠肌肉萎缩与肠道菌群及代谢物变化相关性研究 |
| 投稿时间：2025-05-20 修订日期：2025-07-05 点此下载全文 |
| 引用本文：沈佳敏,唐珊珊,陈琳,潘凌,朱小楠,游利.老年小鼠肌肉萎缩与肠道菌群及代谢物变化相关性研究[J].医学研究杂志,2025,54(12):53-59 |
| DOI：
10.11969/j.issn.1673-548X.2025.12.010 |
| 摘要点击次数: 26 |
| 全文下载次数: 19 |
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| 基金项目:国家重点研发计划资助项目(2021YFC2501700)；上海老龄化和妇儿健康研究专项(2020YJZX0104) |
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| 中文摘要:目的 探讨老年小鼠肌肉萎缩的潜在机制,重点关注肠道菌群组成及代谢物变化的影响。方法 选取20月龄雄性C57BL/6J小鼠作为老年组(n=10),6周龄雄性C57BL/6J小鼠作为年轻组(n=10)。检测肌肉萎缩基因(Atrogin-1和MuRF-1)的表达水平,并通过苏木精-伊红染色染色评估肌肉组织病理变化。利用16S rRNA测序分析肠道菌群组成,采用非靶向代谢组学分析血清代谢物变化。通过京都基因与基因组百科全书通路分析筛选显著差异代谢物及相关代谢通路。结果老年组小鼠的肌肉萎缩程度显著高于年轻组,表现为Atrogin-1和MuRF-1基因表达水平升高,肌肉横截面积和肌纤维数量显著减少。老年组小鼠肠道菌群物种丰富度降低,放线菌门丰度显著升高,而拟杆菌门、糖细菌门和软壁菌门丰度显著降低。老年组小鼠的代谢物组成显著改变,嘌呤代谢和咖啡因代谢通路显著下调。结论 本研究揭示了老年小鼠肌肉萎缩与肠道菌群失调及代谢物变化之间的潜在关联,为肌肉萎缩相关的病理机制提供了新的实验证据。 |
| 中文关键词:肌肉萎缩 肌少症 肠道菌群 代谢组学 |
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| Correlation between Muscle Atrophy and Changes in Gut Microbiota and Metabolites in Aged Mice. |
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| Abstract:Objective To explore the potential mechanisms of muscle atrophy in aged mice, focusing on the impact of changes in gut microbiota composition and metabolites. Methods 20-month-old male C57BL/6J mice were selected as the aged group (n=10), and 6-week-old male C57BL/6J mice were selected as the young group (n=10). The expression levels of muscle atrophy-related genes (Atrogin-1 and MuRF-1) were detected, and muscle tissue pathological changes were assessed using hematoxylin-eosin staining. The gut microbiota composition was analyzed by 16S rRNA sequencing, and serum metabolite changes were analyzed using untargeted metabolomics. Kyoto Encyclopedia of Genes and Genomes pathway analysis was employed to screen significantly altered metabolites and related metabolic pathways. Results The degree of muscle atrophy in the aged group was significantly higher than that in the young group, characterized by increased expression levels of Atrogin-1 and MuRF-1, and significant reduction in muscle cross-sectional area and muscle fibers number. The gut microbiota species richness in the aged group was decreased, with the abundance of Actinobacteria significantly increased, and the abundance of Bacteroidetes, Saccharibacteria, and Tenericutes significantly decreased. The metabolite composition of the aged group was significantly altered, and the purine metabolism and caffeine metabolism pathways were significantly downregulated. Conclusion This study reveals a potential association between muscle atrophy in aged mice and gut microbiota dysbiosis as well as metabolite changes, providing new experimental evidence for the pathological mechanisms of muscle atrophy. |
| keywords:Muscle atrophy Sarcopenia Gut microbiota Metabolomics |
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