| 慢性应激致肠易激综合征小鼠模型的建立与评价 |
| 投稿时间：2025-07-18 修订日期：2025-08-02 点此下载全文 |
| 引用本文：张珊,望庐山,李佳,吴云辰.慢性应激致肠易激综合征小鼠模型的建立与评价[J].医学研究杂志,2025,54(12):88-92, 111 |
| DOI：
10.11969/j.issn.1673-548X.2025.12.015 |
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| 基金项目:湖北省自然科学基金资助项目(面上项目)(2023AFB689) |
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| 中文摘要:目的 采用慢性应激法建立肠易激综合征(irritable bowel syndrome, IBS)小鼠模型,并评价模型的吻合性。方法选取SPF级健康雄性7周龄C57小鼠,将其分为空白组和模型组,每只小鼠单笼饲养,空白组小鼠不做处理,模型组小鼠给予8周的联合应激刺激。对比两组小鼠的一般情况、体质量增长、粪便含水率及粪便性状。通过结肠排珠实验观察结肠动力,糖水偏好实验、旷场实验观察小鼠的抑郁焦虑情绪,腹部撤回反射观察小鼠的内脏敏感度。采用苏木精-伊红(hematoxylin-eosin, HE)染色观察小鼠的结肠组织形态,甲苯胺蓝染色观察结肠组织肥大细胞数量及活化程度,透射电镜观察结肠超微结构。采用酶联免疫吸附试验(enzyme-linked immunosorbent assay, ELISA)检测各组小鼠血清中白细胞介素-1β(interleukin-1β, IL-1β)、肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)、5-羟色胺(5-hydroxytryptamine, 5-HT)、P物质(substance P, SP)以及结肠中5-HT、SP的水平。结果 与空白组比较,模型组小鼠体质量增长缓慢,粪便含水率明显升高,结肠动力下降,抑郁焦虑情绪明显,内脏敏感度升高,结肠组织出现低度炎症,肥大细胞数量增加,超微结构显示细胞明显水肿,血清IL-1β、TNF-α、5-HT、SP水平升高(P＜0.05)。结论 通过慢性应激法联合孤养可以导致小鼠胃肠功能障碍、内脏高敏感度及抑郁焦虑情绪,模拟IBS临床症状,可作为一种有效的IBS动物模型制备方式。 |
| 中文关键词:慢性应激 肠易激综合征 结肠 动物模型 行为学 |
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| Establishment and Evaluation of A Mouse Model of Irritable Bowel Syndrome Induced by Chronic Stress. |
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| Abstract:Objective To establish a mouse model of irritable bowel syndrome (IBS) using chronic stress methods, and the consistency of the model was evaluated. Methods Healthy male C57mice of SPF grade at 7 weeks of age were selected and divided into the blank group and the model group, with each mouse housed individually, Mice in the blank group were not treated, while mice in the model group underwent combined stress stimulation for 8 weeks. The general condition, body weight gain, fecal moisture content, and fecal characteristics of the two groups of mice were compared. Colon motility was observed through a colonic bead expulsion test, and depression and anxiety were assessed using a sugar water test and an open field test. The visceral sensitivity of the mice was observed by abdominal withdrawal reflex. The morphology of the colon tissue of mice was observed by hematoxylin-eosin (HE) staining, the number and activation degree of mast cells in the colon tissue were observed by toluidine blue staining, the ultrastructure of the colon was observed by transmission electron microscopy. Enzyme-linked immunosorbent assay was used to detect the levels of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), 5-hydroxytryptamine(5-HT), substance P (SP)in the serum of mice in each group, as well as the levels of 5-HT and SP in the colon. Results Compared with the blank group, mice in the model group exhibited slow weight gain, significantly increased fecal moisture content, decreased colonic motility, pronounced depressive and anxious emotions, and heightened visceral sensitivity, low-grade inflammation in the colonic tissue, increased number of mast cells, and obvious cellular edema as revealed by electron microscopy ultrastructure, elevated serum levels of IL-1β, TNF-α, 5-HT and SP (P<0.05). Conclusion Chronic stress combined with isolation rearing can induce gastrointestinal dysfunction, visceral hypersensitivity, and depression and anxiety in mice, mimicking the clinical symptoms of IBS, and can be served as an effective way to prepare animal model for IBS. |
| keywords:Chronic stress Irritable bowel syndrome Colon Animal model Behavioral science |
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