| 参附注射液对失血性休克大鼠肾脏血栓调节蛋白及内皮细胞蛋白C受体基因表达的影响 |
| 投稿时间：2009-08-24 点此下载全文 |
| 引用本文：周小洁、潘景业、陈洁、吴双华、张近波、王卫、沈晔.参附注射液对失血性休克大鼠肾脏血栓调节蛋白及内皮细胞蛋白C受体基因表达的影响[J].医学研究杂志,2010,39(1):58-61 |
| DOI：
|
| 摘要点击次数: 1385 |
| 全文下载次数: 1214 |
|
| 基金项目:温州市科技局基金资助项目(Y20060062) |
|
| 中文摘要:目的探讨参附注射液对失血性休克大鼠肾脏血栓调节蛋白（TM）及内皮细胞蛋白C受体（EPCR）基因表达的影响及可能的机制。方法成年健康SD大鼠50只，随机分为5组：正常对照组、实验对照组、失血性休克组、林格液复苏组、参附复苏组，每组10只。建立失血性休克大鼠模型。林格液组用3倍失血量的林格液复苏；参附组用含参附注射液（10ml/kg）和林格液组成的3倍失血量的液体复苏。休克后4h及复苏后3h处死大鼠，留取肾组织，用RT-PCR法检测TM及EPCR的基因表达。结果与正常对照组比较，实验对照组肾组织TM和EPCR的mRNA表达升高（P<0.05），休克后明显升高（P<0.01）；林格液组和参附组复苏后肾组织TM和EPCR的mRNA表达较休克组均下降，差异有统计学意义，林格液组下降更明显（P<001）；林格液组与参附组比较差异有统计学意义（P<0.05）。结论失血性休克时大鼠肾脏组织TM和EPCR表达增加，这与休克时存在内皮细胞功能损伤有关，参附注射液可以从基因水平影响大鼠肾组织TM和EPCR的mRNA表达，保护内皮细胞功能。 |
| 中文关键词:失血性休克、血栓调节蛋白、内皮细胞蛋白C受体、参附注射液 |
| |
| Study on Effects of Shenfu Injection on Thrombomodulin and Endothelial Protein C Receptor on Kindney in Hemorrhagic Shock Rats |
|
|
| Abstract:ObjectiveTo investigate the effects of Shenfu injection on thrombomodulin (Tm) and endothelial protein C receptor (EPCR) on kindney and to clarify its possible mechanism in hemorrhagic shock rats.MethodsIn this study, fifty Sprague-Dawley (SD) rats were randomly divided into the following five groups: controls; sham shock; hemorrhagic shock (HS group); resuscitation with Ringer’s solution (RS group); resuscitation with Shenfu injection (SF group) with ten rats per group. The rats in RS group and SF group were subjected to hemorrhagic shock and resuscitation was performed with three times of the volume of shed blood of Ringer’s solution, or three times of the volume of shed blood of fluid containing Shenfu injection (10ml/kg) plus Ringer’s solution. After four hours of shock or three hours of resuscitation, the rats in various groups were executed and the kindneys were taken out.TM and EPCR messenger ribonucleic acid (mRNA) on kindney were determined by reverse transcription polymerase chain reaction (RT-PCR).ResultsCompared to the control group, the level of TM and EPCR mRNA on kindney in the sham shock group increased (P<0.05), and they increased obviously in the shock group (P<0.01). Kindney TM and EPCR mRNA in the RS group and SF group were lower than those in the shock group,and they changed significantly in the RS group (P<0.01). The levels of TM and EPCR mRNA on kindney were lower in the RS group than those in the SF group (P<0.05).ConclusionThe TM and EPCR on kindney increased in hemorrhagic shock rats. It prompted that the endothelial cell was damnified in these rats. The Shenfu injection could effect TM and EPCR mRNA expression on kindney and protect the endothelial cell. |
| keywords:Hemorrhagic shock Thrombomodulin Endothelial protein C receptor Shenfu injection |
| 查看全文 查看/发表评论 下载PDF阅读器 |
