| 渐进性咬合紊乱大鼠咬肌线粒体Ca2+超载及超微结构变化的研究 |
| 投稿时间：2009-12-08 修订日期：2010-01-08 点此下载全文 |
| 引用本文：张婧,熊利峰,刘晓东,于世宾,付玉婷,王美青.渐进性咬合紊乱大鼠咬肌线粒体Ca2+超载及超微结构变化的研究[J].医学研究杂志,2010,39(3):28-31 |
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| 基金项目:国家自然科学基金资助（30540130469，30872870） |
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| 中文摘要:摘要目的研究渐进性咬合紊乱对大鼠咬肌线粒体Ca2+含量及超微结构的影响。方法8周龄雌性SD大鼠50只，实验组（40只）以推第3磨牙向远中移动的方法建立渐进性咬合紊乱，其中10只仅建立咬合紊乱，20只给予含硝苯地平的二甲基亚砜（DMSO）溶液，10只仅给予DMSO溶液；另外10只为操作对照。各组分别于实验开始后1周和4周取材，观察咬肌超微结构，测定咬肌线粒体Ca2+含量。结果各时间点的咬合紊乱组及咬合紊乱给予DMSO组双侧咬肌均出现超微结构紊乱及线粒体Ca2+含量的明显增加（P<0.001）；而咬合紊乱给予硝苯地平组与操作对照组之间无明显差异(P>0.05)，超微结构未观察到明显改变。结论 渐进性咬合紊乱可导致大鼠咬肌运动性损伤，表现为线粒体Ca2+超载及超微结构异常。 |
| 中文关键词:渐进性咬合紊乱 咬肌 超微结构 线粒体 钙离子 |
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| Mitochondria Ca2+ Overload and Ultrastructure Changes in Masseter Muscle of Rats Induced by Experimental Created Disordered Occlusion |
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| Abstract:AbstractObjectiveTo study the effects of the ECDO on the ultrastructure and the mitochondria Ca2+ content of the rat’s masseter muscle. Methods8-week-old female SD rats were adopted for the experiments. First of all, the experimentally created disordered occlusion (ECDO) was established by moving the left maxillary and right mandibular third molars distally about 0.8mm. The rats were randomly assigned to 4 groups, with 10 for operation control, 10 for ECDO, 20 for ECDO + Nifedipine (in dimethyl sulphoxide, DMSO) injection and 10 for ECDO + DMSO injection. The ultrastructure of masseter was observed by transmission electron microscope（TEM） and the contents of mitochondria Ca2+ in masseter muscles were tested in all groups at the time points of 1 week and 4 weeks after operation. ResultsIn ECDO and ECDO + DMSO groups, the mitochondria Ca2+ contents (P< 0.001) increased significantly and the ultrastructure changed obviously. In ECDO + Nifedipine group, however, the ultrastructure and mitochondria Ca2+ contents (P>0.05) were not different from the operation control group. ConclusionECDO could lead to the injury of rat masseter muscle, presenting with the changes of ultrastructure and Ca2+ overload in mitochondria. |
| keywords:Experimental created disordered occlusion Masseter muscle Mitochondria Ultrastructure Calcium |
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