| TRAIL联合顺铂体外杀伤前列腺癌PC-3细胞株的实验研究 |
| 投稿时间：2009-12-10 修订日期：2010-01-08 点此下载全文 |
| 引用本文：曾四平,肖亚军,章小平,李炎生.TRAIL联合顺铂体外杀伤前列腺癌PC-3细胞株的实验研究[J].医学研究杂志,2010,39(3):40-42 |
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| 基金项目:国家自然科学基金资助项目(30572139) |
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| 中文摘要:摘要目的探讨肿瘤坏死因子相关凋亡诱导配体（TRAIL）联合化疗药物顺铂（DDP）对前列腺癌PC-3细胞株的体外杀伤作用。方法分别采用不同浓度的DDP（1.0μg/ml、5.0μg/ml、10.0μg/ml、20.0μg/ml、50.0μg/ml、100.0μg/ml）与TRAIL（10.0ng/ml、20.0ng/ml、50.0ng/ml、100.0ng/ml、200.0ng/ml）作用PC-3细胞株，24h后MTT法检测细胞的吸光度；DDP(5.0ng/ml)与TRAIL（50.0ng/ml）联合作用PC-3细胞4h、8h、12h、16h、20h、24h后，MTT法检测检测细胞的吸光度；并按细胞的抑制率(100%)=(1-实验组吸光度/阳性对照组吸光度)×100%计算DDP组、TRAIL组及两者联合应用对细胞的抑制率，比较组间细胞抑制率的差异。结果单独应用DDP或者TRAIL对PC-3细胞体外杀伤作用有浓度依赖性，浓度增高一定程度时对PC-3细胞的抑制作用会处于一个相对平台期；联合应用DDP+TRAIL组与单独应用同浓度的DDP及TRAIL组相比，其对PC-3细胞的体外杀伤作用明显增强，P<0.05，差异具有显著性意义；联合应用DDP与TRAIL对PC-3细胞的体外杀伤作用具有明显的时间依赖性。结论DDP能明显提高TRAIL对前列腺癌PC-3细胞株的杀伤作用，其机制与死亡受体DR5的表达上调有关。 |
| 中文关键词:TRAIL 顺铂 DR5 PC-3 |
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| Experiment of Killing Human Prostate Cancer Cell Line (PC-3) with Combination of TRAIL and DDP in vitro |
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| Abstract:AbstractObjectiveTo study the killing human prostate cancer cell line PC-3 with combination of TRAIL and DDP in vitro. MethodsThe PC-3 cell line was separately treated with different concentrations of DDP (1.0μg/ml, 5.0μg/ml, 10.0μg/ml, 20.0μg/ml, 50.0μg/ml, 100.0μg/ml) and TRAIL (10.0μg/ml, 20.0μg/ml, 50.0μg/ml, 100.0μg/ml, 200.0μg/ml) and 24 hours later, cell absorbance was detected by MTT assay. The PC-3 cell line was treated with combination of DDP(5.0μg/ml) and TRAIL（50.0μg/ml）at different time (4h, 8h, 12h, 16h, 20h, 24h) and cell absorbance was detected. The inhibitory rate of cells was calculated as the following:inhibitory rate of cells (100%) = (1-absorbance of experimental group / absorbance of positive control group), and the rates were compared between different groups. ResultsDDP or TRAIL killing PC-3 cells had a dose-dependent manner. When the concentration increased to some extent, the inhibition would be at a relative plateau. As compared with the group of DDP or TRAIL, the killing effect in vitro of DDP and TRAIL in combination increased more (P<0.05), and it had significantly difference. The killing effect of DDP and TRAIL in vitro had an obviously time-dependent manner. ConclusionDDP can significantly enhance TRAIL on prostate cancer PC-3 cell line in vitro. The mechanism is relevant to upregulation of the death receptor DR5. |
| keywords:TRAIL DDP Death receptor5 (DR5) PC-3 |
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