| 支气管肺癌黏膜Cath D、SP-A和GLUT-1的表达及临床意义 |
| 投稿时间：2009-11-30 修订日期：2010-01-09 点此下载全文 |
| 引用本文：曹慧玲,刘师兵,刘晓冬,薛鹏杰,徐冶,刘丹丹.支气管肺癌黏膜Cath D、SP-A和GLUT-1的表达及临床意义[J].医学研究杂志,2010,39(3):70-73 |
| DOI：
|
| 摘要点击次数: 1613 |
| 全文下载次数: 1097 |
|
| 基金项目:吉林省教育厅“十一五”科学技术研究项目（吉教科字2007第244号) |
|
| 中文摘要:摘要目的了解支气管肺癌活组织黏膜组织蛋白酶D(Cath D)、肺表面活性物质相关蛋白A（SP-A）和组织葡萄糖转运蛋白1(GLUT-1)的表达。方法支气管镜下钳取异常组织黏膜41例，经光镜病理确诊为支气管肺癌38例(其中小细胞肺癌10例，鳞状细胞癌28例)，炎症3例。用SP免疫组织化学方法检测Cath D、SP-A和GLUT-1的表达。结果①Cath D：小细胞癌-6例（60%），++4例（40%）；鳞状细胞癌-4例（14%），+2例（7%），++8例（28%），+++14例（50%）。小细胞癌与鳞状细胞癌对Cath D的表达有明显差异（P<0.01），后者明显高于前者。鳞状细胞癌分化程度不同，Cath D的表达有明显差异（P<0.05），分化程度越低，表达越高；②SP-A：+++3例（炎症），++20例（高分化鳞癌15例，中分化鳞癌5例），+6例（中分化鳞癌），-12例（小细胞癌10例，低分化鳞癌2例）；③GLUT-1：小细胞癌-6例，+2例，+++2例；鳞状细胞癌-5例，+7例，++11例，+++5例；炎症+++3例。小细胞癌与鳞状细胞癌对GLUT-1的表达有明显差异（P<0.05），后者明显高于前者；不同分化程度的鳞状细胞癌，GLUT-1的表达无差异（P﹥0.05）；炎症与鳞癌GLUT-1表达有明显差异（P<0.05）与小细胞癌则无差异（P﹥0.05）。结论Cath D有助于鳞状细胞癌的诊断和分化程度的评估，SP-A可能有助支气管黏膜炎症与高恶度肿瘤组织的鉴别，GLUT-1在鳞状细胞癌有高表达，有助于鳞状细胞癌的诊断和鉴别诊断。 |
| 中文关键词:支气管异常黏膜 组织蛋白酶D 肺表面活性物质相关蛋白A 组织葡萄糖转运蛋白1 |
| |
| The Expression of Cath D,SP-A,GLUT-1 of Bronchiogenic Carcinoma |
|
|
| Abstract:AbstractObjectiveTo examine the expression of Cath D, SP-A and GLUT-1 in patients with bronchiogenic carcinoma. MethodsA total of 41 patients were included in the study, 10 of whom received the histological diagnosis of small cell lung cancer (SCLC) .The other 28 were squamous cell carcinoma (SC) and 3 were inflammation. And all the samples were taken from the patients′ tunica mucosa bronchiorum through bronchofibroscope, then we detected the cathepsin D, SP-A and GLUT-1 following SP immunohistochemistry. Results①Cath D: 6 samples (60%) in group SCLC were negtive expression (-6), 4 (40%)were moderately positive (++4), 4 (14%) were negative (-4), 2 (7%) were positive (+2), 8 (28%) were moderately positive (++8) and 14 (50%) were intensive positive (+++14) in the other group. SCLC was significant different from SC in expressing cathepsin D (P<0.01), and the latter group was obviously exceeded with the former one. At the same time, the level of expression depended on the degree of the squamous cell differentiation (P<0.05);②SP-A：+++ for 3 (inflammatory), ++ for 20(15 were high differentiation squamous cell carcinoma; others were middle differentiation squamous cell carcinoma),+ for 6 (middle differentiation squamous cell carcinoma) and - for 12(10 were small cell carcinoma and 2 were poorly differentiated squamous cell carcinoma) were found;③GLUT-1:strong positive was 10 including 3 inflammatory and 7 well differentiated squamous cell carcinoma; positive was 11 (squamous cell carcinoma); weakly positive was 9 (2 of them were small cell carcinoma, others were quamous cell carcinoma); negative was 6(small cell carcinoma).ConclusionThe detection of cathepsin D is of great clinical significance in diagnosing and evaluating differentiation-degree in squamous cell carcinoma. The SP-A may contribute to identification of the tissue belong to bronchial mucosa. The high expression of GLUT-1 in SC is of great clinical significance in tumor diagnosis. |
| keywords:Bronchiogenic carcinoma Cathepsin D Pulmonary surfactant-associated protein-A (SP-A) Glucose transporter-1（GLUT-1） |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
