| 经前期综合征肝气逆、郁两证大鼠模型血清及不同脑区单胺类神经递质含量分析 |
| 投稿时间：2010-01-20 点此下载全文 |
| 引用本文：巢玉彬,魏盛,乔明琦,王杰琼,张惠云.经前期综合征肝气逆、郁两证大鼠模型血清及不同脑区单胺类神经递质含量分析[J].医学研究杂志,2010,39(4):19-22 |
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| 基金项目:国家重点基础研究发展计划（973计划）资助课题（2006CB504804）；国家自然科学基金资助课题（90709009）；山东省科技攻关项目课题（20070915193） |
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| 中文摘要:目的借助前期研制成功PMS肝气逆、郁两证大鼠模型检测其血清与不同脑区单胺类神经递质含量，探索“肝失疏泄”微观机制并对模型与中医证型关系进行初步分析。方法挑出动情周期非接受期大鼠40只，随机分为5组：正常对照组、PMS肝气逆证造模组、PMS肝气郁证造模组、PMS肝气逆证造模给药组、PMS肝气郁证造模给药组，肝气逆证大鼠模型以情志刺激为主多因素复合造模法制备，肝气郁证大鼠模型以慢性束缚应激法制备，模型复制成功后肝气逆大鼠模型用经前平颗粒干预，肝气郁证大鼠模型用经前舒颗粒干预。HPLC测定血清及中枢NE、DA、5-HT含量，比较各组差异并进行分析。结果旷场实验中，相较正常对照组大鼠造模后逆模组水平得分、垂直得分及可旷场实验总分均显著增加(P<0.05)，而郁模组大鼠则显著减少(P<0.05)，用药后这一趋势得到明显纠正(P<0.05)。肝气逆证模型大鼠不同脑区NE、DA、5-HT含量均有上升趋势；肝气郁证模型大鼠不同脑区DA含量呈下降趋势，5-HT含量显著上升。外周单胺类神经介质变化趋势多与中枢相反。结论肝疏泄太过所致烦躁易怒等表现可能与边缘叶DA水平上升，下丘脑DA、边缘叶5-HT水平下降密切相关；肝气疏泄不及呈现抑郁萎靡等症状可能与下丘脑NE水平上升，下丘脑、边缘叶DA水平下降，5-HT水平上升有密切关系。据此推测，肝主疏泄可能通过调节机体该类活性物质含量而呈现作用；其功能定位为脑中枢，尤其是下丘脑。 |
| 中文关键词:PMS肝气逆证/肝气郁证 大鼠模型 单胺类神经递质 |
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| Analysis of Monoamine Neurotransmitter Content in Serum and Different Encephalic Regions of PMS Liver-qi Invasion, Depression Rat Models |
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| Abstract:AbstractObjectiveTo detect monoamine neurotransmitter content in serum and different encephalic region by PMS Liver-qi invasion,depression rat models developmented before and to explore the probable mechanism of “Liver Losing Regulating” and to conduct initial analysis on relation of animal model and patterm of syndrome in TCM. Methods40 rats were selected in the period of non-receptive phases and were divided into 5 group randomly:normal control team, PMS Liver-qi invasion model team, PMS Liver-qi depression model team, PMS Liver-qi invasion medication administration team and PMS Liver-qi deression medication administration team. Multiple factor combination method was adopted to prepare PMS Liver-qi invasion rat model based on emotion stimulation and to prepare PMS Liver-qi depression rat model based on chronic constraint stimulation.After modeling successfully, Jingqianping granule was administrated to PMS Liver-qi invasion rat model and Jingqianshu granule to PMS Liver-qi deression rat model. Monoamine neurotransmitter content was detected in serum and different encephalic region using HPLC. ResultsIn open-field experiment, liver-qi invasion rats′ scores were much higher than those of controlled, while liver-qi depression rats′ scores decreased notably, and the trend was retrieved after administration. Liver-qi invasion rats′ NE,DA, 5-HT contents showed an increasing trend, while liver-qi depression rats DA content decreasing and 5-HT content raising obviously in different encephalic region. Peripherymonoamine neurotransmitter showed an opposite change compared with center. ConclusionTantrum appearance induced by Liver-qi regulating exciting maybe related to the increasing of DA level in limbic lobe,the descending of DA level in hypothalamus and 5-HT level in limbic lobe; while depression symptom induced by Liver-qi regulating restrain maybe related to the increasing of NE level in hypothalamus and the descending of DA level but ascending of 5-HT level in hypothalamus and limbic lobe. “Liver Governing Regulating”may show its function through regulating these active substances and its possible function location is brain center,especially hypothalamus. |
| keywords:PMS Liver-qi invasion Liver-qi depression Rat model Monoamine neurotransmitter |
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