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RECK和MMP-9在喉鳞癌中的表达及其临床意义

投稿时间：2009-11-01 点此下载全文

引用本文：常玮,王斌全.RECK和MMP-9在喉鳞癌中的表达及其临床意义[J].医学研究杂志,2010,39(4):73-76

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作者	单位
常玮	山西医科大学第一医院耳鼻咽喉科
王斌全	山西医科大学第一医院耳鼻咽喉科

中文摘要:目的探讨癌基因MMP-9和抑癌基因RECK在喉癌组织中的表达与喉癌临床特征的关系,了解喉癌的生物学行为，并研究两种基因的表达相关性。方法分别采用PV9000免疫组织化学两步法及RT-PCR方法检测38例喉磷癌组织及对应癌旁正常组织中MMP-9 和 RECK蛋白的表达及mRNA转录情况，同时取10例喉乳头状瘤作为对照。结果①MMP-9在喉鳞癌组织中蛋白阳性表达率为92.11%，显著高于癌旁组织中表达（P＜0.01）；而RECK在癌组织中蛋白阳性表达率为6053%，显著低于癌旁组织中表达（P＜0.01）;②MMP-9与RECK在癌组织中的mRNA相对表达量均与癌旁组织中的表达相比有显著差异（P＜0.01）;③MMP-9和RECK在喉鳞癌组织中的表达水平与患者临床分期和肿瘤病理学分级分别呈正相关和负相关。MMP-9在伴颈淋巴结转移组中的表达水平明显高于非转移组，而RECK则正好相反；④MMP-9的基因表达水平与RECK基因表达呈负相关（r=－0.907,P＜0.01）。结论①MMP-9和RECK两因子在喉鳞癌组织中的表达水平与喉鳞癌患者的临床病理参数密切相关；②MMP-9和RECK可能共同参与了喉鳞癌的发生、发展，并与喉鳞癌的浸润及转移有密切关系。

中文关键词:喉鳞癌 MMP-9 RECK 免疫组织化学 RT-PCR

Expression s of Matrix Metalloproteinase-9 and RECK and Its Significance in Carcinoma of Larynx

Abstract:AbstractObjectiveTo investigate the relation between expression of MMP-9 and RECK in laryngeal carcinoma and it′s clinical characteristics and to explore the biological action of laryngeal tumor. Methods38 laryngeal squamous cell carcinoma (LSCC)specimens and normal mucosa were examined for the expression level of MMP-9 and RECK protein by IHC method ( PV9000) and RT-PCR was employed to measure the expression of MMP-9 and RECK mRNA. Ten laryngeal papilloma were used for controls. ResultsThe expression level of MMP-9 protein in carcinoma of larynx was 92.11%, which was significantly higher than that of the adjacent tissues （P＜0.01）.The expression level of RECK protein in carcinoma of larynx was 60.53%, which was significantly lower than that of the adjacent tissues（P＜0.01）. The expression of MMP-9 and RECK mRNA between tumor and adjacent tissues was significantly different（P＜0.01）. The expression of the two cytokines was significantly associated with clinical staging , pathological grading and cervical lymph node metastasis. The expression of RECK genes was negatively correlated with the expressions of MMP-9 in larynx cancer（r=-0.907, P<0.01）. ConclusionThe expression of MMP-9 and RECK in the tissue of larynx squamous carcinoma was significantly correlated with clinicopathological indexes of the tumor. The two cytokines might participate in the occurrence and development of larynx squamous carcinoma.

keywords:Laryngeal carcinoma MMP-9 RECK IHC RT-PCR

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