| 实时荧光定量RT-PCR检测胃癌腹腔冲洗液CK19 mRNA的临床意义 |
| 投稿时间：2010-01-06 修订日期：2010-03-12 点此下载全文 |
| 引用本文：盛贤能,刘宏斌,朱万坤,韩晓鹏,苏琳.实时荧光定量RT-PCR检测胃癌腹腔冲洗液CK19 mRNA的临床意义[J].医学研究杂志,2010,39(5):45-47 |
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| 基金项目:全军医学科学技术研究“十一五”计划课题项目（06MA082） |
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| 中文摘要:目的评估实时荧光定量反转录聚合酶链式反应(real-time fluorescent quantitative reverse transcription-polymerase chain reaction, FQ-RT-PCR) 方法检测胃癌腹腔冲洗液沉渣中CK19 mRNA的表达，探讨其对预测胃癌腹膜微转移的临床意义。方法收集36例胃癌和7例胃良性病变患者腹腔冲洗液沉渣及临床病理资料,分别采用FQ-RT-PCR检测腹腔冲洗液沉渣中CK19 mRNA的表达;HE染色行腹腔冲洗液细胞学( peritoneal lavage cytology,PLC)检查。结果胃癌CK19 mRNA的阳性检出率为(23/36)639%,明显高于PLC(9/36)25.0% (P<0.01) ,PLC检查阳性的9例患者CK19 mRNA检测结果均为阳性；且CK19 mRNA的检测阳性率与浆膜浸润与否、胃癌浸润深度、组织学分化程度、淋巴结转移情况及病期进展有关（P<0.05）。胃良性病变两项检查结果均为阴性。结论FQ-RT-PCR检测胃癌腹腔冲洗液沉渣中CK19 mRNA,可提高检测腹腔游离癌细胞的灵敏性,适用于预测胃癌患者腹膜微转移,有助于指导术后治疗及判断预后。 |
| 中文关键词:实时荧光定量RT-PCR CK19 胃癌 腹腔冲洗液 细胞学检查 |
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| Clinical Significance of FQ-RT-PCR on Detection of CK19 mRNA Expression in Peritoneal Lavage Residues of Gastric Cancer |
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| Abstract:ObjectiveTo evaluate the effect of real-time fluorescent quantitative reverse transcription-polymerase chain reaction (FQ-RT-PCR) on detection of CK19 mRNA expression in peritoneal lavage residues of gastric cancer and to discuss the clinical significance on prediction of peritoneal micrometastasis of gastric cancer. MethodsPeritoneal lavage residues and clinical pathologic data of 36 patients with gastric cancer and 7 patients with benign gastric lesion were collected. CK19 mRNA expression in peritoneal lavage residues was checked through FQ-RT-PCR and peritoneal lavage cytology (PLC) respectively. ResultsCK19 mRNA positive rate of gastric cancer was (23/36) 63.9% which was obviously higher than that of PLC (9/36) 25.0% (P<0.01). CK19 mRNA results of the 9 patients with positive PLC result was all positive. CK19 mRNA positive rate was related to serosa infiltration, tumor infiltration depth, histopathological differentiation, lymph node metastasis and illness progress (P<0.05). The two results of benign gastric lesion were both negative. ConclusionThe detection of CK19 mRNA expression in peritoneal lavage residues of gastric cancer through FQ-RT-PCR could enhance the sensibility of peritoneal free cancer cells. FQ-RT-PCR is suitable for predicting peritoneal micrometastasis of patients with gastric cancer, which is good for guiding postoperative treatment and evaluating prognosis. |
| keywords:Real-time fluorescent quantitative polymerase chain reaction CK19 Gastric cancer Peritoneal lavage Cytology examination |
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