| 紫七软肝片含药血清调控人肝细胞凋亡相关基因的研究 |
| 投稿时间：2009-12-25 修订日期：2010-03-14 点此下载全文 |
| 引用本文：陈云,蒋凤荣.紫七软肝片含药血清调控人肝细胞凋亡相关基因的研究[J].医学研究杂志,2010,39(5):89-92 |
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| 基金项目:南京中医药大学中医内科开放课题（zynk009） |
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| 中文摘要:目的研究紫七软肝片含药血清对乙醇造模的人肝细胞（LO2）抑制的影响及凋亡相关基因的调控，探讨其对LO2保护作用的分子机制。方法以紫七软肝片灌胃，制备兔含药血清，用乙醇建立LO2凋亡模型，采用MTT法测定含药血清对乙醇致LO2细胞抑制的影响，Hoechest3325荧光检测细胞凋亡形态学变化；Real-time RT PCR 和Western-Blot检测凋亡促进基因casepase3、NF-κB表达。结果紫七软肝片含药血清可调控casepase3、NF-κB的表达，减少乙醇造模的LO2的凋亡。结论紫七软肝片可以通过调控凋亡促进基因casepase3, NF-κB的表达，起到保护LO2的作用。 |
| 中文关键词:紫七软肝片 人肝细胞株 血清药理学 细胞凋亡 基因 |
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| Effect of Serum containing Ziqi Ruangan Pill on Apoptosis Related Genes in LO |
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| Abstract:ObjectiveTo study the influence of serum containing Ziqi Ruangan Pill on inhibitory effect of alcohol LO2 and to investigate the regulation on apoptosis related genes and the protective mechanism for LO2. MethodsRabbit sera saline containing Ziqi Ruangan Pill was prepared. The apoptotic LO2 model was established by being cultured with alcohol. The effect of serum on the model was detected by MTT method. Identification of apoptosis was carried out using flow cytometry and Hoechst3325 fluorescent staining. The expression of apoptotic inducing gene, casepase3 and NF-κB was detected by Real-time RT PCR and Western-Blot. ResultsThe serum containing Ziqi Ruangan Pill could regulate the expression of the casepase3，NF-κB and reduce the apoptosis. ConclusionZiqi Ruangan Pill can protect the LO2 by regulating the expression of apoptosis stimulative gene casepase3 and NF-κB. |
| keywords:Ziqi Ruangan Pill LO2 Serum pharmacology Cell apoptosis Gene |
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