| 中药水蛭素对荷瘤鼠Ki-67与VEGF表达的影响 |
| 投稿时间：2010-01-18 修订日期：2010-04-05 点此下载全文 |
| 引用本文：任青华,牟忠祥,耿延君,张博,李焱,任海全.中药水蛭素对荷瘤鼠Ki-67与VEGF表达的影响[J].医学研究杂志,2010,39(6):46-48 |
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| 基金项目:山东省中医药现代化重大科技专项基金(2008GG2NS02023)、山东省自然科学基金（Y2006C62） |
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| 中文摘要:目的分析Ki-67增生抗原与VEGF细胞因子在荷瘤鼠组织中的表达，探讨其与肿瘤的内在关系。方法取小鼠48只，随机分成4组，左前腋下接种H22肝癌瘤细胞0.2毫升/只。连续用药水蛭素，第15天处死动物，剥离肿块称重。同时取荷瘤鼠肿块组织固定、包埋、切片、染色、孵育、滴加抗体等，用免疫组化法检测Ki-67增生抗原与VEGF细胞因子的表达。Ki-67阳性表达定位于细胞核，VEGF阳性表达定位于细胞质。结果Ki-67与VEGF模型对照组呈强阳性；水蛭素大剂量组呈弱阳性；水蛭素小剂量组呈阳性。结论水蛭素能抑制荷瘤鼠肝癌实体瘤Ki-67增生抗原与VEGF细胞因子的高表达，具有明显的抗肿瘤作用，这与中药水蛭素活血化瘀及强大的抗凝血功效有密切关系。 |
| 中文关键词:中药水蛭素 H22肝癌实体瘤 Ki-67增生抗原 VEGF细胞因子 |
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| The Effect of CMH on the Expression of Ki-67 Gene and VEGF in Mice |
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| Abstract:ObjectiveTo analyze the expression of Ki-67 proliferation antigen and the VEGF in the tumor-bearing mice organization，and to explore its intrinsic relationship with the tumor. Methods48 mice were randomly divided into four groups.0.2ml of H22 liver tumor cell was transplanted into the left armpit of every mice.CMH was used continuously, and animals were killed at the 15th days.Then tumour was stripped and weighed.The tumor-bearing mice′s tumour organization was simultaneously taken to be fixed, embeded, sliced, dyed, fostered, added by antibody.The expression of Ki-67gene and VEGF was detected by immunohistochemistry. The positive expression of Ki-67 was located in the nucleus,and VEGF expression in the cytoplasm. ResultsKi-67 and VEGF showed strong positive realation in model control group,showed weak positive in high-dose hirudin group,and showed positive in low-dose group of hirudin. ConclusionHirudin can inhibit the high expression of Ki-67 proliferation antigen and VEGF of the tumor-bearing mice liver cancer solid tumor.CMH has obvious inhibition of tumor, which has the direct relation with CMH's Huoxuehuayu and powerful anti-clotting effect. |
| keywords:CMH H22 liver cancer solid tumor Ki-67 proliferation antigen VEGF |
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