| 检测甲型H1N1流感病毒富集方法的初步探索 |
| 投稿时间：2010-01-29 修订日期：2010-04-05 点此下载全文 |
| 引用本文：李华,柯华昕,龙润乡,杨蓉,何春艳,白慧珠,崔萍芳,谢忠平.检测甲型H1N1流感病毒富集方法的初步探索[J].医学研究杂志,2010,39(6):71-73 |
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| 中文摘要:目的探索甲型H1N1流感病毒的富集方法，提高流感病毒核酸检测的灵敏度。方法分别用ProteinA/G及H1N1特异性血清处理流感病毒样品，根据国家流感中心设计的H1N1亚型检测通用引物，采用反转录-聚合酶链反应(RT-PCR)方法，扩增产物为527bp，来评价病毒的富集效果。结果两种富集方法均能提高检测灵敏度，用ProteinA/G富集处理后检测灵敏度可达10-6，用H1N1特异性血清沉淀富集处理后灵敏度为10-5。结论初步建立的富集甲型H1N1流感病毒方法，有效提高了流感病毒RT-PCR检测的灵敏度，可用于甲型H1N1流感病毒的检测。 |
| 中文关键词:流感病毒 甲型H1N1亚型 富集 检测 |
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| he Primary Exploration of Enrichment Methods for Influenza A (H1N1) Virus |
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| Abstract:ObjectiveTo explore the enrichment methods for influenza A (H1N1) virus and improve the detection sensitivity of the virus nucleotide. MethodsThe influenza A (H1N1) virus samples were enriched by Protein A/G and the specific serum respectively.Universal primers of H1N1 subtype, which is designed by the National Influenza Center were applied in RT-PCR method to evaluate the effect of the virus enrichment. The RT-PCR product was 527bp. ResultsThese two enrichment methods both could improve the detection sensitivity. The detection sensitivity of Protein A/G and H1N1-specific serum enrichment methods could reach 10-6 and 10-5 respectively. ConclusionSuch established enrichment methods for influenza A (H1N1) virus could effectively improve the detection sensitivity of influenza viruses through RT-PCR and could be used to detect Influenza A (H1N1) virus. |
| keywords:Influenza virus H1N1 subtype Enrich Detect |
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