| 同型半胱氨酸对基质金属蛋白酶的影响 |
| 投稿时间：2010-01-15 修订日期：2010-04-05 点此下载全文 |
| 引用本文：陈妍,马静萍.同型半胱氨酸对基质金属蛋白酶的影响[J].医学研究杂志,2010,39(6):88-90 |
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| 摘要点击次数: 1143 |
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| 中文摘要:目的观察高同型半胱氨酸血症（HHcy)大鼠基质金属蛋白酶-2，基质金属蛋白酶-9（MMP-2、MMP-9）的变化，探讨HHcy致动脉粥样硬化（As）的机制。方法30只Wistar雄性大鼠随机分为对照组，蛋氨酸组及干预组。对照组给予普通饲料，蛋氨酸组普通饲料中添加2%蛋氨酸喂养，干预组普通饲料中添加2%蛋氨酸喂养同时每日给予叶酸0.5mg，维生素B12（VitB12 ）25μg灌胃，实验期为8周。检测血清Hcy、MMP-2和MMP-9。用HE染色法对颈总动脉进行病理学检查。结果干预组和蛋氨酸组血清Hcy、MMP-2、MMP-9浓度均高于对照组(P＜0.05)，干预组血清Hcy、MMP-2、MMP-9浓度均低于蛋氨酸组（P＜0.05）；蛋氨酸组MMP-2和MMP-9与Hcy呈正相关。镜下观蛋氨酸组颈总动脉内膜平滑肌细胞增生排列紊乱，弹力纤维融合。结论HHcy诱导MMP-2和MMP-9表达增加，可能是HHcy致As的机制。可以通过补充维生素B12和叶酸来预防HHcy，从而防止As的发生。 |
| 中文关键词:高同型半胱氨酸血症 动脉粥样硬化 MMP-2 MMP-9 叶酸 维生素B12 |
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| Effects of Homocysteine on Matrix Metalloproteinase |
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| Abstract:ObjectiveTo observe the expression of matrix metalloproteinases-2，metalloproteinases-9,(MMP-2,MMP-9) in hyperhomocysteinemia(HHcy) rats,and to investigate the mechanism of atherosclerosis(AS) induced by HHcy. MethodsThirty male Wistar rats were randomly divided into 3 groups: control group,in which rats was fed with normal diet; methionine group,in which rats was fed with normal diet plus 2% methionine;and intervention group,in which rats was fed with normal diet plus 2% methionine and 0.5mg folate, 25μg vitamin-B12（VitB12 ）as daily supplementation for 8 weeks. Serum levels of homocysteine(Hcy), MMP-2，MMP-9 were assessed.The pathological change of carotid artery was observed. ResultsCompared with control group, the levels of Hcy, MMP-2，MMP-9 were significantly higher in methionin group and intervention group(P＜0.05). Compared with methionin group, the levels of Hcy, MMP-2，MMP-9 were significantly lower in intervention group(P＜0.05). There were hyperplasia and derangement of smooth muscle, syzygium of elastic fibers.ConclusionIncreasing expression of MMP-2 and MMP-9 induced by HHcy may be one of the pathogenesies of atherosclerosis. VitB12 and folate supplementation can prevent HHcy,and the occurrence of AS. |
| keywords:Hyperhomocysteinemia Atherosclerosis Matrix metalloproteinases-2 Matrix metalloproteinases-9 Folate VitaminB12 |
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