| 精子透明质酸酶活性与精液动态参数的相关性 |
| 投稿时间：2010-03-27 点此下载全文 |
| 引用本文：杨建辉,高晓勤,陈利红.精子透明质酸酶活性与精液动态参数的相关性[J].医学研究杂志,2010,39(8):59-62 |
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| 基金项目:贵州省省长专项基金（黔省专合字2007 46号）；贵州省科技厅攻关项目（黔科合S字20071030）；黔南州科技项目（黔南科合2008 5） |
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| 中文摘要:目的探讨不育男性精子顶体内透明质酸酶活性与精液有关参数的关系，以期为临床男性不孕不育的病因分析、诊断、治疗及精子的功能检查提供理论及实验依据。方法收集81例精液标本，其中正常生育对照组为20例，不育男性61例，以精子质量分析仪对每份标本进行计算机辅助精液分析（CASA）。并利用改良明胶底物膜法检测精子顶体内透明质酸酶活性。结果①不育组与对照组相关动态参数的分析结果：VCL、VSL、VAP、LIN、WOB的数据不育组与对照组具有统计学意义，表明不育组精子综合质量与对照组间存在差异;②精子运动活力的参数即精子的VCL、VSL、VAP以及ALH 等精子参数与精子顶体内透明质酸酶活性有显著相关性，与LIN、STR、WOB、BCF及STR无统计学意义。结论①不育组与对照组相关动态参数VCL、VSL、VAP、LIN、WOB数据具有统计学意义，精子综合质量与对照组间存在差异;②精子参数VCL、VSL、VAP及ALH与精子顶体内透明质酸酶活性有显著相关性。 |
| 中文关键词:精子透明质酸酶 精液参数 关系 |
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| Relationship between Sperm Acrosin Hyaluronidase Activity and Conventional Semen Parameters |
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| Abstract:ObjectiveTo study the relationship between sperm acrosin hyaluronidase activity in infertilitle men and conventional semen parameters in order to provide theoretical and experimental basis for analysis of cause, diagnosis, treatment of clinical male infertile, and for sperm function tests. MethodsEighty-one semen samples were collected. Twenty cases were in normal control group, and other cases were infertile men (excluding the infertility caused by women ). Conventional clinical examination of each semen specimen was made by using sperm quality analysis instrument and computer-assisted sperm analysis (CASA). Sperm hyaluronidase activity was detected by using the method of modified gelatin membrane. Results①There was significant difference in the related dynamic parameters of VCL, VSL, LIN and WOB between the infertile men and control persons which indicated that sperm quality was unnormal. ②Sperm motility parameters (VCL、VSL、 VAP以及ALH) were related significantly with sperm hyaluronidase activity. There was no statistical significance in LN，STR，WOB，BCF and STR between the two groups. Conclusion①The infertile group and control group showed statistical significanc in relevant dynamic parameters such as VCL, VSL, VAP, LN and WOB. The integrated quality of the sperm in infertile group is different from that of control group.②There is a significant correlation between the sperm parameters(VCL, VSL,VAP,ALH) with sperm acrosin hyaluronidase activity. |
| keywords:Sperm Hyaluronidase Semen Parameters Relation |
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