| HCV抗体阳性样品核酸及核心抗原检测分析 |
| 投稿时间：2010-07-13 点此下载全文 |
| 引用本文：龙润乡,杨蓉,白慧珠,李华,王晶晶,廖芸,谭振国,谢忠平.HCV抗体阳性样品核酸及核心抗原检测分析[J].医学研究杂志,2010,39(12):41-43 |
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| 基金项目:国家“863”资助项目(2007AA02Z480);云南省联合支持国家计划资助项目(2008GA008) |
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| 中文摘要:目的对90份临床检测为丙型肝炎病毒抗体（HCV-Ab）阳性样品检测其核心抗原（HCV-cAg）、荧光定量PCR，观察3者间的相关性。方法采集临床诊断为HCV-Ab阳性样品90份，分别用市售的HCV-cAg检测试剂盒、荧光定量PCR 检测试剂盒、HCV-Ab检测试剂盒进行3种指标的检测复核。结果90份临床HCV-Ab阳性样品，检出HCV-cAg阳性 25份（27.8％），荧光定量PCR检出67份阳性（74.4％），抗体复核阳性的75份（83.33％）；检出率HCV-Ab（83.33％）＞荧光定量（74.4％）＞HCV-cAg（27.8％）。结论丙肝的3种标志物之间存在一定的联系，但不同试剂的检测结果间存在明显差异。 |
| 中文关键词:丙型肝炎病毒核心抗原 丙型肝炎病毒核酸 丙型肝炎病毒抗体 检测 |
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| HCV RNA and Core Antigen (HCV-cAg) Analysis in HCV-Ab Positive Samples |
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| Abstract:ObjectiveClinical samples of 90 confirmed HCV-Ab positive cases were detected for core antigen (HCV-cAg) and HCV RNA by fluorescence quantitative PCR (FQ-PCR), and the relativity of the three was observed. Methods90 samples diagnosed as HCV-Ab positive cases were collected, and the three indicators was detected and rechecked with HCV-cAg ELISA kit, HCV-Ab ELISA Kit and quantitative hepatitis C virus(HCV) PCR Fluorescence Diagnostic kit for sale in market separately. ResultsAmong 90 clinical samples of HCV-Ab positive cases, HCV-cAg was positive in 25 cases (27.8%), FQ-PCR was positive in 67 cases (744%), and antibody rechecking was positive in 75 cases (83.33%). As regards to the positive rate, HCV-Ab was the highest and florescence quantitative took the second place, and HCV-cAg was the lowest. ConclusionIt had some relations among the three makers of HCV, but there were significant differences in detection results of different agents. |
| keywords:HCV core antigen(HCV-cAg) HCV-RNA HCV-Ab Detection |
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