| miR-221和miR-222在急性髓细胞白血病初发患者中的表达研究 |
| 投稿时间：2010-11-14 点此下载全文 |
| 引用本文：李宝青,薛向阳,夏轶姿,朱宝玲,何牧卿,李向阳,张丽芳.miR-221和miR-222在急性髓细胞白血病初发患者中的表达研究[J].医学研究杂志,2011,40(6):110-113 |
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| 中文摘要:目的探讨miR-221和miR-222在急性髓细胞白血病（AML）骨髓有核细胞中的表达及意义。方法选择16例AML初发患者和44例非白血病患者骨髓标本，分离有核细胞，抽提总RNA，以U6为内参，采用茎环Realtime RT-PCR法检测并比较AML和非白血病患者骨髓有核细胞中miR-221和miR-222的表达，同时比较这两种miRNAs在AML中M3、M4和M5三种亚型间的表达水平。结果miR-221和miR-222在AML初诊患者骨髓中相对表达量（N=2-ΔCt）明显高于非白血病患者组（P<0.05）；这两种miRNAs在M5型AML患者表达最高，但在AML三种亚型间表达水平未见显著的统计学意义（P>0.05）。结论miR-221和miR-222有可能在为AML的诊断、治疗和预后上，提供一个新的标准和靶点指标。 |
| 中文关键词:miR-221 miR-222 急性髓细胞白血病 |
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| The Expression and Significance of miR-221 and miR-222 in the Acute Myeloid Leukemia |
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| Abstract:ObjectiveTo investigate the expression and clinical significance of miR-221 and miR-222 in the marrow of acute myeloid leukemia(AML). MethodsMarrow tissues from 16 newly diagnosed AML patients and 44 non-leukemia (NL) patients were collected and mononucleated cell were separated.Total RNA from nucleated cells of these samples were extracted and analyzed for miR-221 and miR-222 expression by stem-loop real-time RT-PCR. The differences in subtypes M3, M4 and M5 patients and the change of miR-221 and miR-222 expression in newly diagnosed AML patients were further analyzed. ResultsExpression levels of miR-221 and miR-222 were significantly higher in marrow tissues of AML patients before chemotherapy than the levels in those of non-leukemia patients (P<0.05). Both miR-221 and miR-222 expression in the M5 of AML were the highest among them, but this difference in subtypes M3, M4 and M5 patients was not statistically significant（P>0.05）. ConclusionmiR-221 and miR-222 may play an important role in the development and progression of AML and the level of miR-221 and miR-222 expression may be a potential target for the diagnosis, treatment and prognosis of AML. |
| keywords:MicroRNA miR-221 miR-222 Acute myeloid leukemia |
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