| 阿仑膦酸钠对破骨细胞分化中c-fos基因表达的影响 |
| 投稿时间：2010-11-14 修订日期：2011-05-13 点此下载全文 |
| 引用本文：王立,戚孟春,刘彩霞,董伟.阿仑膦酸钠对破骨细胞分化中c-fos基因表达的影响[J].医学研究杂志,2011,40(7):71-74 |
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| 基金项目:河北省科学技术研究与发展计划项目(08276101D -73) |
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| 中文摘要:目的探讨阿仑膦酸钠对破骨细胞分化中c-fos基因的作用机制。方法将不同浓度的阿仑膦酸钠作用于小鼠破骨细胞，采用Western blot检测小鼠破骨细胞分化中c-fos基因的表达情况。结果阿仑膦酸钠组TRAP阳性破骨细胞数目和c-fos基因的表达显著低于空白对照组；且加入10-4mol/L、10-6mol/L、10-8mol/L浓度阿仑膦酸钠后，c-fos表达受抑制程度依次增强；而在10-8mol/L、10-10mol/L、10-12mol/L浓度中，c-fos表达受抑制程度依次减弱。结论阿仑膦酸钠可使破骨细胞分化中的c-fos基因受到抑制，其抑制程度与阿仑膦酸钠的浓度有关，10-8mol/L浓度时抑制作用最强。 |
| 中文关键词:阿仑膦酸钠 c-fos 破骨细胞 Western blot |
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| Abstract:ObjectiveTo study the probably mechanism of alendronate sodium to c - fos gene in osteoclast differentiation. MethodsDifferent concentrations of alendronate sodium had effect on mice osteoclast. c-fos expression in mouse osteoclast differentiation was detedcted using Western blot. ResultsThe number of TRAP+ osteoclasts and the expression of c-fos gene from alendronate group were significantly lower than those in control group (P<0.05), and the expression of c-fos was enhanced with the concentration increase of alendronate in 10-4mol/L,10-6mol/L,10-8mol/L, but the expression of c-fos was decreased with the concentration increase of alendronate in 10-8mol/L,10-10mol/L,10-12mol/L. ConclusionAlendronate sodium can make osteoclast differentiation of c-fos gene tobe restrained. Its inhibition degree was associated with alendronate sodium concentration.The strongest inhibition effect on c-fos expression was at concentration of 10-8mol/L。 |
| keywords:Alendronate sodium c-fos Osteoclast Western blot |
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