| 新疆和田维吾尔族长寿老人永生细胞株的建立 |
| 投稿时间：2011-01-12 修订日期：2011-01-24 点此下载全文 |
| 引用本文：袁晓华,吐尔洪·克维尔,穆斯塔帕,左宏莉,乔艳辉,郭伟鹏.新疆和田维吾尔族长寿老人永生细胞株的建立[J].医学研究杂志,2011,40(9):81-83 |
| DOI：
|
| 摘要点击次数: 1121 |
| 全文下载次数: 914 |
|
| 基金项目:乌鲁木齐市科技局人才工程项目编号（P09131005） |
|
| 中文摘要:目的通过用EB 病毒转化外周血B 淋巴细胞使之成为永生细胞株的方法,建立新疆和田维吾尔族长寿老人永生细胞株，为永久保存新疆长寿老人的基因遗传资源及后续研究奠定基础。方法常规制备EB病毒上清，用环孢素法和冻存全血法转化外周血B淋巴细胞。结果以环孢素法成功建立了5株新疆和田维吾尔族长寿老人永生细胞，其中百岁2人，90~99岁3人，建系成功率为100%（5/5），以冻存全血法建系成功1例（1/5）。HLA基因分型结果在建系前后完全一致。结论与冻存全血法相比，环孢素法能更有效地转化长寿老人永生细胞株，可为长寿的分子遗传机制研究以及某些细胞生物学性状的检测提供充足的实验材料。 |
| 中文关键词:EB病毒 永生细胞株 维吾尔族长寿老人 和田 |
| |
| Establishment of Immortalized B Lymphoblast Cell Lines from the Longevous Uygur People in HeTian XinJiang |
|
|
| Abstract:ObjectiveTo establish immortal cell lines from the longevous Uygur people in HeTian.MethodsWe used two methods of transforming human B lymphocyte into immortal lymphoblast cell lines: cyclosporine method and frozen whole blood method.ResultsBoth of the methods can be used to establish the cell lines successfully.Cyclosporine method (5/5) was more efficient than frozen whole blood method (1/5).Five immortal longevity cell 1ines were obtained successfully，which included 2 centenarians and 3 nonagenarians.The results of HLA genotyping showed the genetic stability of these immortal cell lines.ConclusionThese immortal B lymphoblast cell lines would preserve the unique genome of longevous Uygur people in Hetian.It could provide adequate experimental material for the exploration of the origin and molecular mechanism of human longevity. |
| keywords:Epstein-Barr virus Immortal B lymphoblastoid cell lines Longevous Uygur peopl Hetian |
| 查看全文 查看/发表评论 下载PDF阅读器 |
