| 重组SRH蛋白冻干保护剂的研究 |
| 投稿时间：2011-03-04 修订日期：2011-03-16 点此下载全文 |
| 引用本文：邹民吉,徐涛,刘深,付文亮,徐东刚.重组SRH蛋白冻干保护剂的研究[J].医学研究杂志,2011,40(11):25-27 |
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| 基金项目:国家科技重大专项、重大新药创制课题（2009ZX09103-623） |
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| 中文摘要:目的筛选并优化重组SRH蛋白的冻干保护剂，使其能够保持良好的生物学活性。方法选择不同浓度的冻干保护剂，包括甘露醇、右旋糖酐40、蔗糖和甘氨酸等组成不同浓度的单一和复合配方，冷冻干燥后检测冻干样品的溶栓活性和成型的变化，同时，冻干样品经过37℃加速实验后，检测其溶栓活性和成型状况的改变，对比分析各组保护剂的效果。结果不同浓度的甘露醇和右旋糖苷40对SRH蛋白的保护作用良好，没有明显差异，而不同浓度的蔗糖和甘氨酸保护效果较差。复方中以右旋糖苷40、蔗糖和甘胺酸的复合对目的蛋白的保护作用最好；不同浓度的甘露醇、蔗糖和甘氨酸的复合应用无明显的保护作用。结论以右旋糖苷40、蔗糖和甘氨酸组成的复合配方对重组SRH蛋白的冻干过程有明显的保护作用。 |
| 中文关键词:重组SRH蛋白 冻干保护剂 生物活性 |
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| Study on Lyophilized Protective for Recombinant Anti-artherosclerosis Protein on the Basis of SAK Backbone |
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| Abstract:ObjectiveTo select a lyophilized protective for recombinant anti-artherosclerosis protein on the basis of SAK backbone. MethodsMannitol, dextran 40, sucrose,and glycine in their different concentrations were selected to make up a single recipe or different complex recipes of their concentrations. After lyophilization, the thrombolytic activity and plasticity change should be examined, in the meantime, the lyophilized sample was rapidly thowed at 37℃ and the changes of thrombolytic activity and plasticity also should be examined.ResultsThe protective effects on the protein of interest were not significantly different between glyceral and dextran 40 in their different concentrations. Both of them possessed better protective effect, however, that of the sucrose and glycine in different concentrations were less effective. The complex recipe of dextran 40, sucrose and glycine displayed the best protective effect on the protein of interest among the complex recipes, but the complex of gyceral, sucrose and glycine in the different concentrations had no obvious protective effect. ConclusionThe dextran 40, sucrose and gycine in differet concentrations possess a significantly protective effect on the lyophilized process of the recombinant anti-artherosclerosis protein on the basis of SAK backbone. |
| keywords:Recombinant anti-artherosclerosis protein Lyophilized protective Biological activity |
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