| 异丙酚自身给药对大鼠伏隔核内ERK表达的影响 |
| 投稿时间：2011-05-05 修订日期：2011-05-17 点此下载全文 |
| 引用本文：王本福,杨博,孙安娜,黄茜茜,金胜威,李军,赖苗军,周文华,刘惠芬,张富强,连庆泉.异丙酚自身给药对大鼠伏隔核内ERK表达的影响[J].医学研究杂志,2011,40(11):39-41 |
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| 基金项目:国家自然科学基金资助项目（30972840/C16）；浙江省自然科学基金资助项目（Z2101211）;温州市科技局项目（Y20100046） |
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| 中文摘要:目的观察异丙酚静脉自身给药对大鼠伏隔核内p-ERK表达的影响。方法SD大鼠随机分为3组（n＝6）：对照组（C组）、1.00mg/kg异丙酚组（P1组）、1.70mg/kg异丙酚组（P2组）。用静脉自身给药法建立异丙酚精神依赖模型，Western blotting法检测大鼠伏隔核内p-ERK和ERK的变化。结果P1组、P2组的异丙酚可以诱发大鼠建立静脉自身给药行为。与P1组相比，P2组大鼠有效鼻触（P＜0.01）和注射次数（P＜0.01）明显增加。随着异丙酚剂量的增加，大鼠伏隔核内p-ERK/ERK的表达明显增加（P＜0.01）。结论异丙酚静脉自身给药增加了大鼠伏隔核内p-ERK的表达，ERK信号转导通路可能参与了异丙酚的精神依赖性。 |
| 中文关键词:异丙酚 精神依赖性 细胞外信号调节激酶 自身给药 伏隔核 |
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| Effect of Propofol Maintained Self-administration on Expression of ERK in Nucleus Accumbens |
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| Abstract:ObjectiveTo investigate the effect of propofol maintained self-administration on the expression of p-ERK in nucleus accumbens of rats. MethodsRats were divided into 3 groups (n=6): intralipid control group and propofol groups (P1=1.00mg/kg, P2=1.70mg/kg). To assess the psychic dependence of propofol, the technique of intravenous self-administration by rats was utilized. The expression of p-ERK/ERK in NAc was detected by Western blotting. ResultsAll the rats trained with propofol at dose 1.00mg/kg and 1.7mg/kg showed self-administration behavior. Compared to P1 group, the number of active nose-poke response and injections of P2 group increased significantly (P<0.01). Further, changed of propofol, the expression of p-ERK/ERK in nucleus accumbens was dose-dependent increased (P<0.01). ConclusionThe data suggested that propofol-maintained self-administration increase the expression of p-ERK in NAc, and the ERK signal Pathway maybe involve in psychic dependence of propofol. |
| keywords:Propofol Psychological dependence ERK Self-administration NAc |
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