| FOLFOX方案化疗对胃癌术后患者外周血CD4+CD25+调节性T细胞细胞增殖及功能的影响 |
| 投稿时间：2011-03-14 修订日期：2011-04-07 点此下载全文 |
| 引用本文：蒋平,唐湘莲,韩绍伟.FOLFOX方案化疗对胃癌术后患者外周血CD4+CD25+调节性T细胞细胞增殖及功能的影响[J].医学研究杂志,2012,41(3):61-65 |
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| 基金项目:温州市科技局科研科技合作资助项目（2008S0411） |
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| 中文摘要:目的探讨FOLFOX方案化疗对胃癌术后患者外周血CD4+CD25+调节性T细胞(Treg细胞)比例及功能的影响。方法选择43例胃癌术后化疗患者，于化疗前1天、化疗结束后第5天采集外周血。采用流式细胞法检测外周血Treg细胞的比例；RT-PCR法检测外周血Treg细胞FoxP-3 mRNA表达水平；将分选的Treg细胞和CD4+CD25-T细胞按单纯Treg细胞组、1∶1混合细胞组，单纯CD4+CD25-T细胞组培养，3H-TdR掺入法检测Treg细胞体外抑制CD4+CD25-T细胞增殖的能力。结果43例胃癌患者化疗前1天外周血Treg细胞占CD4+T细胞的比例为17.63%±6.31％，化疗后降至13.79%±4.82％(P<0.01)；化疗后FoxP-3表达水平较化疗前显著下降(P<0.01)；化疗后Treg细胞对CD4+CD25-T细胞的增殖抑制率由化疗前的5464%±5.15%下降至24.63%±2.34%(P<0.01)。结论FOLFOX化疗方案可减少胃癌患者外周血Treg细胞数量，下调其表面标志物FoxP3基因的表达水平，减弱Treg细胞的免疫抑制功能，有利于诱生抗肿瘤免疫效应。 |
| 中文关键词:胃癌 CD4+CD25+ 调节性T细胞 化疗 |
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| Influence of FOLFOX Chemotherapy on the Percentage and Function of CD4+CD25+ Regulatory T Cell in Peripheral Blood of Gastric Cancer Patients after Operation |
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| Abstract:ObjectiveTo observe the influence of FOLFOX chemotherapy on the percentage and function of CD4+CD25+ regulatory T cell(Treg) in peripheral blood(PB) of gastric cancer patients after operation. MethodsThe prevalence of Treg in PB one day before chemotheraphy and five days after chemotheraphy was examined by flow cytometry method in 43 cases patients suffering gastric cancer. RT-PCR techniques were used to detect the expression of FoxP3 mRNA in these Treg cells. To determine regulatory properties, Treg(freshly isolated) and CD4+CD25- T cell from tumor patients were cocuhured in a ratio of 1∶1. Proliferation was measured by 3H-TdR assay. ResultsThe percentage of cells in the PB of 32 patients before chemotheraphy was 17.63%±6.31％ in CD4+T cells, and the percentage was decreased to 13.79%±4.82％ after chemotheraphy(P<0.01). The FoxP3 mRNA expression of Treg cells was significantly decresed after chemotheraphy (P<0.01).The inhibitory rate of Treg cells on CD4+CD25- T cell after chemotherapy was 24.63%±234%, which down from 54.64% ± 5.15% before chemotherapy(P<0.01). ConclusionFOLFOX chemotherapy in patients with gastric cancer can reduce the number of Treg cells, reduce the expression levels of their surface markers FoxP3 mRNA, reduce the function of Treg cells in immune suppression, which is beneficial for inducing anti-tumor immune response. |
| keywords:Gastric cancer CD4+CD25+ Regulatory T cell Chemotherapy |
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