| DEHP诱导的氧化应激致小鼠学习记忆能力的下降和维生素E的保护作用 |
| 投稿时间：2011-08-28 修订日期：2011-09-05 点此下载全文 |
| 引用本文：程静菲,尚帅,何贵波,刘晓帆,唐佳琦,熊国梅,廖晓梅,杨旭.DEHP诱导的氧化应激致小鼠学习记忆能力的下降和维生素E的保护作用[J].医学研究杂志,2012,41(5):24-28 |
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| 基金项目:国家自然科学基金面上资助项目（51076079）；华中师范大学2011年大学生科研立项资助项目 |
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| 中文摘要:目的研究邻苯二甲酸二乙基己酯（Di-2-ethylhexyl phthalate，DEHP）对小鼠学习记忆能力的影响与其在脑组织造成氧化损伤的关系。方法36只雄性昆明小鼠随机分成4组，每组9只，分别为空白对照组（生理盐水）、DEHP染毒组（50mg/kg DEHP）、维生素E（vitamin E）保护组 (50mg/kg DEHP +50mg/kg 维生素E）、维生素E对照组(50mg/kg 维生素E)。每天清晨灌胃1次，连续10天。期间进行Morris水迷宫测试，检验小鼠的学习记忆能力变化，10天后将小鼠处死，取出脑组织，检验ROS及MDA含量。结果Morris水迷宫结果显示，DEHP染毒组小鼠学习记忆能力与对照组相比显著下降(P<0.05)，而维生素E作为抗氧化剂在一定程度上可减少其下降幅度； DEHP染毒组小鼠脑组织中的ROS水平和MDA含量显著上升(P<005)，而维生素E可减少这种增加，并与对照组没有显著差异。结论维生素E作为抗氧化剂可对DEHP致小鼠学习记忆能力损伤有保护作用，DEHP致小鼠学习记忆能力损伤可能与其在脑组织造成的氧化损伤有关。 |
| 中文关键词:邻苯二甲酸二乙基己酯 维生素E 学习 记忆 Morris水迷宫 氧化损伤 |
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| Decreased Learning and Memory Ability Caused by the Oxidative Stress Induced by DEHP and the Protection Effect of Vitamin E in Mice |
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| Abstract:ObjectiveTo study the impairment effect of DEHP on the abilities of learning and memory in KM mice and discuss its relationship with oxidative damage induced by DEHP in the brain. MethodsThirty-six male KM mice were randomly divided into 4 groups (n=9 for each group), including control group (Saline), DEHP treatment group (50mg/kg DEHP), vitamin E protection group (50mg/kg DEHP +50mg/kg VitE), VitE control group (50mg/kg VitE). With the constant exposure for ten days, the Morris water maze was used to test the abilities of learning and memory. Then the reactive oxygen species (ROS) level and malondialdehyde (MDA) content of brains were measured.ResultsThe results of MWM showed that the mice only treated with DEHP had a poorer learning and memory ability compared with the control group (P<0.05 ), and VitE could protect the mice from the impairment of DEHP. The DEHP group had a significantly higher ROS and MDA level in their brains compared with the other three groups, and VitE reduced its increase. Meanwhile, the VitE control group had no significant difference.ConclusionThese results suggested that VitE could protect Kunming mice from the learning and memory impairment induced by the DEHP, which might result from the oxidative damage in their brain. |
| keywords:Di-(2-ethylhexyl)phthalate (DEHP) Vitamin E Learning Memory Morris water maze (MWM) Oxidative damage |
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