| AQPs在宫颈鳞癌癌变过程中的表达及意义 |
| 投稿时间：2011-06-27 修订日期：2011-09-14 点此下载全文 |
| 引用本文：林温静,邹双微,朱雪琼,屈王蕾,江娜.AQPs在宫颈鳞癌癌变过程中的表达及意义[J].医学研究杂志,2012,41(5):105-109 |
| DOI：
|
| 摘要点击次数: 992 |
| 全文下载次数: 920 |
|
|
| 中文摘要:目的研究水通道蛋白（aquaporins，AQPs）在正常宫颈组织、宫颈上皮内瘤样变（CIN）和宫颈鳞癌中的表达，分析其与宫颈鳞癌临床病理资料的相关性，探讨其在宫颈鳞癌病变中的可能作用及意义。方法免疫组化检测47例宫颈鳞癌、37例CIN、16例正常宫颈组织中AQPs的表达及分布。双重免疫组化染色法检测宫颈鳞癌中AQP1与VEGF，AQP3与VEGF，AQP8与VEGF的共同表达与分布。结果①AQP1在3组宫颈组织间质的微血管内皮细胞中表达;②AQP3、AQP4、AQP5和AQP8在正常宫颈鳞状上皮细胞、CIN异形细胞及宫颈鳞癌癌细胞胞质和（或）胞膜中表达；③AQP1在CIN的表达高于正常宫颈组织和宫颈鳞癌（P＜0.05），在临床Ⅰ期宫颈鳞癌的表达高于临床Ⅱ期（P＜0.05）；④AQP3、AQP4、AQP5、AQP8在正常宫颈组织、CIN、宫颈鳞癌的表达逐渐增高；⑤AQP3在低分化组宫颈鳞癌中的表达高于中-高分化组（P＜0.05）；⑥AQP1和VEGF，AQP3和VEGF，AQP8和VEGF在宫颈鳞癌中共同表达。结论AQP1、AQP3、AQP4、AQP5和AQP8在宫颈鳞癌癌变过程中起了一定的作用；AQP1、AQP3和AQP8可能参与宫颈鳞癌微血管的形成。 |
| 中文关键词:水通道蛋白 宫颈上皮内瘤变 宫颈鳞癌 微血管 |
| |
| Expression and Role of AQPs During the Progression of Squamous Cervical Carcinoma |
|
|
| Abstract:ObjectiveTo study the expression of AQPs in normal cervical tissue, cervical intraepithelial neoplasia and squamous cervical carcinoma, and analyze the correlations of AQPs with clinicopathological parameters of squamous cervical carcinoma and explore their possible role during progression to squamous cervical carcinoma. MethodsImmunohistochemical staining technology was applied to detect the expression and position of AQPs in 16 cases of normal cervical tissues, 37 cases of cervical intraepithelial neoplasia tissues and 47 cases of squamous cervical carcinoma specimens. Double immunohistochemistry was used to detect the co-expression of VEGF and AQP1, AQP3, AQP8 in squamous cervical carcinoma. ResultsThe expression of AQP1 was demonstrated in microvascular endothelial cells of the three groups of cervical tissue. The expression of AQP3, AQP4, AQP5 and AQP8 were detected in cytoplasm and (or) membrane of squamous cells in cervical normal tissue, atypia cells in cervical intraepithelial neoplasia and carcinoma cells in squamous cervical carcinoma. AQP1 was highly expressed in the CIN when compared with normal cervical tissue and squamous cervical carcinoma (P＜0.05). In the cases of squamous cervical carcinoma, AQP1 expressed in FIGO stage Ⅰ was higher than that in stage Ⅱ(P＜0.05). The expression of AQP3, AQP4, AQP5 and AQP8 was gradually increasing as the tumor progresses. AQP3 was highly expressed in the poorly-differentiated group as compared with moderately- and well- differentiated group (P＜0.05). Co-expression of the VEGF and AQPs (including AQP1, AQP3 and AQP8) were detected in squamous cervical carcinoma. ConclusionAQP1, AQP3, AQP4, AQP5 and AQP8 may play certain roles during the progression of squamous cervical carcinoma, while AQP1, AQP3 and AQP8 may play a role in the angiogenesis of squamous cervical carcinoma. |
| keywords:Aquaporins Cervical intraepithelial neoplasia Squamous cervical carcinoma Microvascular |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
