| TCF7L2在2型糖尿病大鼠胰岛中的表达及影响 |
| 投稿时间：2014-09-09 修订日期：2014-09-16 点此下载全文 |
| 引用本文：桂书彦,韩瑾,叶强,李静,陈立波.TCF7L2在2型糖尿病大鼠胰岛中的表达及影响[J].医学研究杂志,2015,44(2):133-137 |
| DOI：
10.3969/j.issn.1673-548X.2015.02.037 |
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| 中文摘要:目的 研究2型糖尿病大鼠模型中Wnt通路关键基因转录因子7类似物2(TCF7L2)的表达。方法 利用小剂量链脲佐菌素(STZ, 25mg/kg)联合高脂高糖喂养诱导建立2型糖尿病大鼠模型, 成模后处死并取大鼠胰腺组织, 镜下观察胰岛形态学改变, 免疫组化检测胰岛β细胞的增殖、凋亡, 免疫荧光和Western blot法检测TCF7L2和β-catenin表达。结果 12周后检测糖尿病模型组中大鼠血糖较正常对照组明显升高, 体重变化不明显。免疫组化检测糖尿病模型组胰岛β细胞增殖减少, 凋亡增加, 免疫荧光及Western blot法检测均提示糖尿病模型组中胰岛细胞TCF7L-2表达明显增多。结论 Wnt信号通路中的关键效应基因TCF7L2可能通过参与调节胰岛β细胞的增殖和凋亡途径, 在糖尿病的发病机制中起着重要作用。 |
| 中文关键词:转录因子7类似物2 Wnt信号通路 Beta细胞 糖尿病 |
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| Effect and Expression of TCF7L2 in Diabetic Rat Model |
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| Abstract:Objective To investigated the role of TCF7L2 which is the key effector of Wnt signaling in streptozocin(STZ) diabetic rat model. Methods Type 2 diabetic male Wistar rats was made with injections of 25 mg/kg streptozotocin and 8 weeks of the high-carbohydrate/high-fat diet.2weeks later, plasma glucose and body weight were measured. Morphological observation was performed by light microscopic analyses.Islet cell apoptosis and proliferation were determined by TUNEL and Ki67+immunohistochemical staining. Additionally, the level of TCF 7L2 and beta-catenin expressed in pancreatic islets were also studied by immunofluorescence and western blot analysis, respectively. Results After 16 weeks treatment, the blood glucose was significantly elevated in STZ-induced diabetic rats. The number of Ki67+proliferating β-cells was decreased, whereas the apoptoticβ-cells as determined by TUNEL assay was elevated. Furthermore, immunofluorescence and western blot analysis of the pancreas revealed that the diabetic rat model had an increased expression of TCF7L2. Conclusion The increased activity and expression of TCF7L2 may be one of the mechanism of T2DM. |
| keywords:Transcription factor 7-like 2(TCF7L2) Wnt signaling Diabetes mellitus |
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