| 百里醌诱导膀胱癌细胞凋亡作用机制的研究 |
| 投稿时间：2014-08-24 修订日期：2014-09-16 点此下载全文 |
| 引用本文：王大文,朱诗建,木海琦,李晟,周鹏飞,杨森,南存金,陈映鹤.百里醌诱导膀胱癌细胞凋亡作用机制的研究[J].医学研究杂志,2015,44(3):93-96 |
| DOI：
10.3969/j.issn.1673-548X.2015.03.026 |
| 摘要点击次数: 1407 |
| 全文下载次数: 900 |
|
| 基金项目:浙江省中医药科技计划基金资助项目(2011ZB092);浙江省卫生厅基金资助项目(2012KYB134) |
|
| 中文摘要:目的 探讨百里醌对人膀胱癌细胞株BIU-87细胞凋亡的影响及对其诱导凋亡的潜在作用机制。方法 应用CCK8法检测细胞增殖活性, Hoechst33258染色法检测细胞凋亡, 荧光定量PCR检测基因Bcl-2、Bax、caspase-3、c-myc mRNA的表达水平, Wsetern blot法检测不同浓度百里醌作用后Bcl-2、Bax、c-myc蛋白表达水平的变化。结果 细胞增殖抑制曲线结果显示百里醌能明显抑制BIU-87细胞增殖, 其24、48、72h半数抑制浓度(IC50)分别是38.75±0.58、34.33±1.01和32.43±0.71μmol/ L。百里醌能以剂量依赖性方式诱导膀胱癌细胞凋亡。百里醌作用于BIU-87细胞后, Bcl-2、c-myc mRNA及Bcl-2、c-myc蛋白的表达量呈浓度依赖性降低, 而caspase-3、Bax mRNA及caspase-3、Bax蛋白表达水平呈浓度依赖性递增。结论 百里醌能抑制BIU-87细胞的增殖, 且能诱导BIU-87细胞的凋亡, 其诱导凋亡机制可能与Bcl-2、c-myc表达水平降低及caspase-3、Bax表达水平上调有关。 |
| 中文关键词:百里醌 膀胱癌 Bcl-2 Bax c-myc caspase |
| |
| Effects of Thymoquinone on Inducing Bladder Cancer BIU-87 Apoptosis |
|
|
| Abstract:Objective To investigate the effect of thymoquinone(TQ) on the human bladder cancer cell lines (BIU-87) apoptosis and its possible mechanisms of inducing apoptosis. Methods Cell proliferation ability was calculated by cell counting kit-8(CCK8). Hoechst staining was used to observe the apoptosis of BIU-87 cells, treated with different concentrations of TQ. The mRNA expressions of Bcl-2, Bax, c-myc and caspase-3 were detected by real time fluorescent quantitative PCR. The expressions of Bcl-2, bax and c-myc proteins were determined by Western blot. Results The results showed that TQ remarkably inhibited the BIU-87 cell proliferation with the IC50 of 24h、48h and 72h respectively were 38.75±0.58, 34.33±1.01, 32.43±0.71μmol/L. Apoptosis was induced after treated with TQ. Compared with the blank control group, the mRNA and proteins expressions of Bcl-2 and c-myc obviously decreased in a dose-dependent manner, while the expressions of bax and caspase-3 were upregulated. Conclusion TQ could significantly inhibit the proliferation ability of BIU-87 cells, and also induce apoptosis probably by upregulating the expressions of Bax and caspase-3 and downregulating the expressions of Bcl-2 and c-myc. |
| keywords:Thymoquinone Bladder cancer Bcl-2 Bax C-myc Caspase |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
