| 慢性给予GHRP-6对小鼠跑轮运动日节律的影响 |
| 投稿时间：2014-10-08 修订日期：2014-10-18 点此下载全文 |
| 引用本文：郝维,周岚,谷婧丽,曹济民.慢性给予GHRP-6对小鼠跑轮运动日节律的影响[J].医学研究杂志,2015,44(5):24-27 |
| DOI：
10.11969/j.issn.1673-548X.2015.05.007 |
| 摘要点击次数: 1281 |
| 全文下载次数: 970 |
|
| 基金项目:国家自然科学基金资助项目(81071072,31471126) |
|
| 中文摘要:目的 本研究探讨慢性给予GHRP-6对小鼠日运动节律的影响。方法 利用MiniMitter 鼠类跑轮活动监测系统实时记录C57BL6/J小鼠在全黑暗条件下跑轮运动的昼夜节律;在不同时间点(CT6、CT12、CT14、CT18、CT22和CT24)每日腹腔注射GHRP-6(100μg/kg),观察GHRP-6对小鼠跑轮运动昼夜节律和运动活性的影响。结果 在CT12 给予GHRP-6引起明显的跑轮运动日节律时相延迟,而在其他时间点不引起明显的时相位移。GHRP-6同时引起一定程度的运动活性降低,但该效应与给药时间没有明显关系。结论 GHRP-6可引起C57BL6/J小鼠的跑轮运动日节律时相的时间依赖性延迟和时间非依赖性运动活性降低,该研究提示GHSR信号通路对生物钟有调节作用。 |
| 中文关键词:生长激素释放肽 生长激素促泌素受体 昼夜节律 相移 生物钟 |
| |
| Effects of Chronic GHRP-6 Administration on the Circadian Wheel-running Rhythm in Mice |
|
|
| Abstract:Objective To investigate the effect of GHRP-6 on the circadian locomotor rhythm of mice. Methods The circadian wheel-running activities of C57BL6/J mice were monitored with the MiniMitter Data Acquisition System under constant darkness (DD). GHRP-6(100μg/kg)was injected (ip) daily for 14 days at different circadian times (CT6,CT12,CT14,CT18,CT22 and CT24). Results Repeated GHRP-6 injection at CT12 induced significant phase delay of the circadian rhythm, but not at other CTs. Repeated GHRP-6 injection also suppressed the activity intensities, but this effect was not strictly time-dependent. Conclusion GHRP-6 can induce time-dependent phase delay of the circadian rhythms and time-independent suppression of activity intensities in mice. The study suggests that GHSR signaling has a regulatory role in the circadian rhythm. |
| keywords:Growth hormone releasing peptides Growth hormone secretagogue receptor Circadian rhythm Phase shift Biological clock |
| 查看全文 查看/发表评论 下载PDF阅读器 |
