| 卡介苗感染人B细胞诱导细胞凋亡 |
| 投稿时间：2014-12-25 修订日期：2015-01-04 点此下载全文 |
| 引用本文：朱旗,冯国栋,杨毅宁,赵青,王文菁,张敏,刘扬,赵钢.卡介苗感染人B细胞诱导细胞凋亡[J].医学研究杂志,2015,44(5):37-40 |
| DOI：
10.11969/j.issn.1673-548X.2015.05.010 |
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| 基金项目:国家自然科学基金资助项目(81371334) |
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| 中文摘要:目的 探究卡介苗(BCG)是否能感染人B细胞及观察感染后对细胞的影响。方法 人B细胞系Raji细胞与BCG共培养, 4、12和24h后分别用共聚焦及透射电镜观察Raji细胞对BCG的结合和摄取情况,用CCK-8法检测细胞毒性,流式Annexin Ⅴ-PI双标法检测细胞凋亡和坏死情况。结果 培养4、12和24h后,感染了BCG的细胞比例分别为12.4%±2.0%, 23.8%±5.1%, 25.2%±4.8%。 BCG感染可引起细胞毒性,4h后Raji细胞存活率为75.5%±8.8%,12h后细胞存活率降至51.0%±5.3%,24h后细胞存活率仅为21.6%±4.2%,而感染灭活BCG的细胞存活率均在95%以上。进一步用流式检测凋亡坏死发现,与未感染细胞相比,Raji细胞感染BCG后凋亡,坏死均有所增加,以凋亡增加为主。结论 BCG可以感染人B细胞并诱导细胞凋亡。 |
| 中文关键词:卡介苗 B细胞 Raji细胞 感染 凋亡 |
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| BCG Infection to Human B Cells Induce Cell Apoptosis |
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| Abstract:Objective To explore whether Bacillus Calmette-Guérin (BCG) can infect human B cells and observe the effects of the infection on B cells. Methods Human B cell line Raji cells were incubated with BCG. After incubation for 4h, 12h and 24h, the direct interaction of Raji cells with BCG was observed under confocal microscopy and transmission electronic microscopy. Cytotoxity was detected by cell counting kit (CCK-8). Cell apoptosis and necrosis were assayed by flow cytometry. Results After 4h, 12h and 24h of incubation, the percentage of Raji cells that were infected by BCG was 12.4%±2.0%, 23.8%±5.1%, 25.2%±4.8%, respectively. Live BCG infection induced cytotoxicity. The cell survival rates at 4h, 12h, 24h were 75.5%±8.8%, 51.0%±5.3%, 21.6%±4.2% respectively, while the cells infected with inactivated BCG showed a high survival rate of 95% at any time. Further analysis by flow cytometry showed that cell apoptosis and necrosis, predominantly cell apoptosis of Raji cells were increased by BCG infection. Conclusion BCG can infect human B cells and induce cell apoptosis. |
| keywords:Bacillus Calmette-Guérin(BCG) B cells Raji cells Infection Apoptosis |
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