| 弗氏完全佐剂致大鼠急性膝关节炎动物模型制备 |
| 投稿时间：2014-11-28 修订日期：2014-12-17 点此下载全文 |
| 引用本文：陈德森,李莉,吴胜英,彭吉霞,李贤玉.弗氏完全佐剂致大鼠急性膝关节炎动物模型制备[J].医学研究杂志,2015,44(8):152-155 |
| DOI：
10.11969/j.issn.1673-548X.2015.08.044 |
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| 基金项目:湖北医药学院研究生启动基金资助项目(2008QDJ9) |
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| 中文摘要:目的 复制符合临床急性膝关节炎病理生理特征的实验性大鼠急性膝关节炎动物模型。 方法 30只大鼠随机分为对照组、醋酸组、佐剂组,分别于第1、3、7天经右膝关节腔内注射生理盐水、醋酸及弗氏完全佐剂0.5ml/d,并于造模前、造模后7、14天分别采用ELISA法测定膝关节腔积液中白细胞介素-1β(IL-1β)、透明质酸(HA)含量;测量右膝关节及以下部位的体积,并计算各组动物膝关节肿胀百分率。于14天处死动物取膝关节滑膜组织做病理学镜检。 结果 造模后对照组关节无肿胀现象,醋酸组大鼠右膝关节及其周围肿胀,舔足跛行,模型成功率为80%;佐剂组僵硬肿胀等症状更加明显,模型成功率达100%,肿胀百分率明显高于醋酸组,造模第7、14天关节积液检查显示IL-1β明显升高,HA含量降低,与造模前及对照组、醋酸组比较,差异有统计学意义(P结论 弗氏完全佐剂可成功建立大鼠急性膝关节炎动物模型且优于醋酸法,更符合临床急性膝关节炎病理生理特征。 |
| 中文关键词:大鼠 膝关节炎 白细胞介素-1β 透明质酸 膝关节肿胀百分率 |
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| Animal Model Preparation of Rat's Acute Knee Arthritis Induced by Freund's Complete Adjuvant |
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| Abstract:Objective To copy the animal model of experimental rats in accordance with clinical acute knee arthritis pathophysiological characteristics. Methods Thirty rats were randomey divided into control group, acetic acid group, adjuvant group, respectively. On the first day (d), 3 d, 7 d after the right knee joint cavity injection of saline, acetic acid and Freund's completed adjuvant 0.5 mL/d. Interleukin-1 beta (IL-1 β) in knee joint fluid was measured by enzyme-linked immunosorbent assay(ELISA), and hyaluronic acid (HA) content was measured by biochemistry in 7 and 14 days of postoperation. The volume of right knee joint and below parts was measured and the percentage of swelling of knee joint in each group was calculated. At the7 of 14th day, the animals were killed, synovial tissue of knee joint were taken and determined by pathology. Results After modeling, control group had no joint swelling, Rats in the acetic acid group licked foot and walked with a limp, and their right knee joint and surrounding were swelled. The success rate of the model was 80%. The stiff symptom of swelling in adjuvant group was more obvious and the percentage of swelling was significantly higher than that of acetate group. The success rate of the model was up to 100%. The content of IL-1 beta was significantly increased and HA decreased of the model in joint effusion after seven and fourteen days. There were significant differences compared with control group (P<0.05). There was obvious inflammation in the knee joint of the adjuvant group through pathological section microscopy. Articular cavity had a large effusion. Cartilage surface was rough. Synovitis had a phenomenon of proliferation, adhesion and hypertrophy. It was consistent with typical clinical pathology of knee arthritis. Conclusion It was better to induce acute knee arthritis rat animal model with Freund's complete adjuvant than acetic acid. It conformed more to the clinical pathophysiological characteristics of acute arthritis of the knee. |
| keywords:Rat Knee osteoarthritis Interleukin-1β Hyaluronic acid Knee joint swelling percentage |
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