| 大孔吸附树脂纯化天麻眩晕宁合剂水提部位的工艺研究 |
| 投稿时间：2015-04-04 修订日期：2015-05-12 点此下载全文 |
| 引用本文：柳小莉,曹瑞,曹林林,郭东艳.大孔吸附树脂纯化天麻眩晕宁合剂水提部位的工艺研究[J].医学研究杂志,2015,44(12):67-69,74 |
| DOI：
10.11969/j.issn.1673-548X.2015.12.018 |
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| 基金项目:陕西省教育厅基金资助项目(2012JC11) |
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| 中文摘要:目的优选天麻眩晕宁合剂水提部位的大孔吸附树脂纯化工艺。方法以天麻素、芍药苷为指标,考察上样药液浓度、上样药液流速、径高比、洗脱剂、洗脱速度等对天麻眩晕宁合剂水提部位的影响,确定大孔树脂纯化工艺条件。结果选用HPD300型号大孔树脂,径高比为1:10,最大上样量为3BV,上样药液浓度为0.5gml/生药,以4BV/h的流速上样,1BV水洗脱后,用5BV 50%乙醇4BV/h进行洗脱。结论该工艺纯化天麻眩晕宁合剂水提部位中天麻素、芍药苷是稳定、可行的。 |
| 中文关键词:大孔吸附树脂 天麻眩晕宁合剂 纯化工艺 天麻素 芍药苷 |
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| Purification Technology of Water-extraction Parts from Tianma Xuanyunning Mixture by Macroporous Resin. |
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| Abstract:Objective To optimize purification technology of water-extraction parts from Tianma Xuanyunning mixture by macroporous resin. Methods The gastrodin and paeoniflorin being taken as index, purification condition of water-extraction parts from Tianma Xuanyunning mixture was determined by selecting the effect of liquid concentration, flow velocity, diameter-height ratio, eluent and eluant rate. Results The optimum purification as follows:HPD300 Macroporous resin was fitted, the diameter-height ratio of resin column of 1:10,the sampling volume of eluting reagent of 1BV water and 5BV 50% ethanol,eluting rate of 4BV/h. Conclusion The optimized method is stable and feasible to purify gastrodin and paeoniflorin of water-extraction parts from Tianma Xuanyunning mixture. |
| keywords:Macroporous resin Tianma Xuanyunning mixture Purification Gastrodin Paeoniflorin |
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