| 外伤导致原发性血小板增多症与血巨核细胞体外扩散的相关性临床分析 |
| 投稿时间：2015-04-11 修订日期：2015-05-25 点此下载全文 |
| 引用本文：何学鹏,杨凯,陈鹏,刘兵,张媛,王芳,郭智,刘晓东,楼金星,陈惠仁,刘更夫.外伤导致原发性血小板增多症与血巨核细胞体外扩散的相关性临床分析[J].医学研究杂志,2015,44(12):109-111,116 |
| DOI：
10.11969/j.issn.1673-548X.2015.12.030 |
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| 基金项目:黄石医药卫生科研基金资助项目(黄科技发农[2014]3号) |
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| 中文摘要:目的探讨外伤导致原发性血小板增多症与血巨核细胞体外扩散的相关性。方法2007年9月~2013年12月选择在笔者医院进行急诊的外伤导致原发性血小板增多症患者36例作为观察组,同期选择在笔者医院进行健康体检的健康志愿者36例作为对照组,两组都抽取静脉血进行血浆TPO与IL-11的检测。分离脐带血的单个核细胞,然后与两组的血浆进行共孵育培养,进行CD41a+的表达情况、巨核祖细胞计数和巨核细胞DNA倍体分析。结果观察组在细胞培养10天与15天的巨核细胞数量明显多于对照组。经过巨核系祖细胞集落检测,结果显示观察组的CFU-MK值明显高于对照组(P<0.05)。观察组的血浆TPO和IL-11值明显高于对照组,同时观察组的培养第10天与第15天的多倍体细胞(n ≥4)的比例明显高于对照组,对比差异有统计学意义(P<0.05)。结论外伤导致原发性血小板增多症伴随有巨核细胞扩散性与生成增加,是由于血浆中TPO和IL-11过表达增加而导致巨核细胞成熟形成多倍体,使得血小板增多。 |
| 中文关键词:外伤 原发性血小板增多症 巨核细胞 体外扩散 |
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| Clinical Correlation Analysis Between the Trauma Induced Essential Thrombocythemia and Megakaryocytes Vitro Proliferation. |
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| Abstract:Objective To investigate the clinical correlation between the trauma induced essential thrombocythemia and megakaryocytes vitro proliferation. Methods We selected 36 trauma induced essential thrombocythemia patients from September 2007 to December 2013 in our hospital as the observation group, and selected 36 cases of healthy volunteers as the control group at the same period. All cases were detected venous blood plasma TPO and IL-11.We separated the cord blood mononuclear cells, which were incubated with the two groups' plasma for detecting CD41a+ expression, megakaryocyte progenitor cell count and megakaryocytic DNA ploidy analysis. Results The number of megakaryocytes in the observation group on the cultured 10 and 15 days were significantly more than the control group. The egakaryocyte progenitor colony assay showed that CFU-MK value of the observation group was significantly higher than the control (P<0.05). The plasma TPO and IL-11 levels in the observation group were significantly higher, while the proportion of polyploid cells (n ≥ 4) in the observation group on the cultured 10 and 15 days were significantly higher, compared to significant differences (P<0.05). Conclusion Trauma induced essential thrombocythemia is more accompanied by megakaryocytes vitro proliferation. It is due to increase TPO and IL-11 overexpression in plasma and increase cell maturation resulting in the formation of polyploid megakaryocytes to thrombocytosis. |
| keywords:Trauma Essential thrombocythemia Megakaryocytes Vitro proliferation |
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