| Sysmex CS-5100与CA-7000全自动血凝仪抗脂血和溶血干扰能力的初步比较 |
| 投稿时间：2015-10-06 修订日期：2015-10-16 点此下载全文 |
| 引用本文：费鲜明,王欢,蒋雷,袁武峰,黄小强,孙书辉.Sysmex CS-5100与CA-7000全自动血凝仪抗脂血和溶血干扰能力的初步比较[J].医学研究杂志,2016,45(4):57-62 |
| DOI：
10.11969/j.issn.1673-548X.2016.04.016 |
| 摘要点击次数: 1301 |
| 全文下载次数: 1115 |
|
| 基金项目:浙江省自然科学基金资助项目(LQ13H190005) |
|
| 中文摘要:目的 比较Sysmex CS-5100和CA-7000全自动血凝仪抗脂血和溶血干扰的能力。方法 以两血凝仪分别测定双水平质控品PT、APTT、Fbg、TT的批内和天间不精密度(CV%)。混合血浆中分别加入终浓度(0~5.0%)甘油三酯(TG)脂肪乳剂和0~32g/L血红蛋白(Hb)溶液,以两台血凝仪测定PT、APTT、Fbg、TT,计算相对偏差(%),偏差以5.0%(PT和APTT)和6.67%(TT和Fbg)为标准。结果 CS-5100和CA-70004项测定的批内CV%为0.77%~1.31%,天间CV%为2.00%~3.31%。TG 0~5.0%范围内,两仪器的PT偏差均<5.0%;CA-7000在Hb 16g/L时PT偏差>5.0%(6.32%),CS-5100在Hb(0~32)g/L范围内PT偏差均<5.0%。CA-7000和CS-5100分别在TG 1.0%和2.0%、Hb 1g/L和2g/L时APTT偏差>5.0%(6.90%和7.66%、-8.74%和-7.69%)。CA-7000和CS-5100分别在TG 1.0%和3.0%、Hb1g/L时TT偏差>6.67%(7.37%和7.25%、8.38%和6.45%)。CA-7000在TG 3.0%时Fbg偏差>6.67%(-7.89%),CS-5100在TG 0~5.0%、两仪器在Hb 0~32g/L时Fbg偏差均<6.67%。各浓度TG和Hb时CA-7000的APTT、TT和Fbg测定偏差均大于CS-5100。结论 Sysmex CS-5100抗脂血和溶血干扰的能力比CA-7000更强。 |
| 中文关键词:CS-5100 CA-7000 脂血 溶血 抗干扰 |
| |
| Comparison of the Ability of Sysmex 5100 and CA7100 Automatic Coagulation Analyzer in Anti-interference from Hemolysis and Lipohemia |
|
|
| Abstract:Objective To compare the ability of Sysmex 5100 and CA7100 automatic coagulation analyzer in anti-interference from hemolytic and lipohemic samples. Methods The within-run and inter-day coefficients of variation (CV%) of PT, APTT, Fbg, and TT were determined with two levels of IQC by CS-5100 and CA7000, respectively. Mixed plasma was mixed with fat emulsion with final concentrations of 0-5.0% triglyceride (TG), and hemoglobin (Hb) solution of 0-32g/L Hb. Then the mixed plasma was measured for the four indicators, and the relative deviation was calculated. The allowed levels of deviation were 5.0% for PT and APTT, and 6.67% for TT and Fbg. Results The within-run and inter-day CV% of PT, APTT, TT and Fbg was 0.77%-1.31% and 2.00%-3.31% for the two analyzers, respectively. At 0-5.0% of TG, the deviation of PT was less than 5.0% for both analyzers; and at 16g/L of Hb, the deviation of PT was beyond 5.0% (6.32%)for CA-7000, but at 0-32g/L of Hb, all deviation of PT was less than 5.0%. The deviation of APTT for CA-7000 and CS-5100 at 1.0% and 2.0% of TG as well as at 1g/L and 2g/L Hb was beyond 5.0% (6.90% and 7.66% as well as-8.74% and 7.69%), respectively. CA-7000 showed two beyond-6.67% deviation of 7.37% and 8.38% at 1.0% of TG and 1g/L of Hb compared with that of 7.25% and 6.45% of CS-5100 at 3.0% of TG and 1g/L Hb, respectively. At 3.0% of TG, the deviation of Fbg for CA-7000 was beyond 6.67% (-7.89%), but CS-5100 at 0-5.0% of TG, and both analyzers at 0-32g/L of Hb, the deviation of Fbg was all less than 6.67%. At different concentration of TG and Hb, the deviation of APTT, TT and Fbg of CA-7000 was all higher than that of CS-5100. Conclusion Sysmex CS-5100 has stronger ability of anti-interference from hemolysis and lipohemia. |
| keywords:Sysmex CS-5100 CA-7000 Lipohemia Hemolysis Anti-interference |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
