| 地黄多糖对乳鼠心肌细胞缺氧/复氧损伤保护作用的研究 |
| 投稿时间：2015-09-08 修订日期：2015-10-11 点此下载全文 |
| 引用本文：孙立峰.地黄多糖对乳鼠心肌细胞缺氧/复氧损伤保护作用的研究[J].医学研究杂志,2016,45(4):146-150 |
| DOI：
10.11969/j.issn.1673-548X.2016.04.038 |
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| 中文摘要:目的 研究地黄多糖(RPS)对乳鼠心肌细胞缺氧/复氧损伤的保护作用。方法 取出生3天的SD大鼠乳鼠分离心肌细胞,培养72h后分为6组:空白对照组、缺氧/复氧模型对照组、地黄多糖(10、20、40μg/ml)干预组和舒血宁注射液(SXN,100μg/ml)干预组,每组设10个复孔。各组细胞经药物干预6h后,通过倒置光学显微镜观察各组细胞形态变化,通过甲基四唑蓝(MTT)比色法测定细胞存活率,通过流式细胞术测定细胞凋亡率;测定各组细胞培养液中心肌酶[谷草转氨酶(AST)、磷酸肌酸激酶(CPK)、乳酸脱氢酶(LDH)]含量;测定各组细胞中抗氧化酶[超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)]活性和丙二醛(MDA)含量。结果 与缺氧/复氧模型对照组相比,地黄多糖干预组心肌细胞形态明显好转,细胞存活率明显升高、凋亡率显著降低,培养液中AST、CPK、LDH含量显著降低,细胞中SOD、GSH-Px、CAT活性显著升高且MDA含量显著降低,其中以地黄多糖40μg/ml干预组效果最为显著,差异具有统计学意义(P<0.05,P<0.01)。结论 地黄多糖能够有效改善缺氧/复氧损伤心肌细胞形态、提高细胞存活率、降低细胞凋亡率、改善抗氧化酶活性、抑制氧化应激损伤,提示地黄多糖对缺氧/复氧损伤心肌细胞具有保护作用。 |
| 中文关键词:地黄多糖 乳鼠 心肌细胞 缺氧/复氧损伤 保护 |
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| Protective Effects of Rehmannia Glutinosa Polysaccha-rides on Cadiocytes of Neonatal Rat Impaired by Hypoxia-reoxygenation |
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| Abstract:Objective To investigate the protective effects of rehmannia glutinosa polysaccha-rides(RPS) on cadiocytes of neonatal rat impaired by hypoxia-reoxygenation(H/R). Methods Cadiocytes of neonate rat were cultivated for 72 hours and divided into six groups:Normal control group, H/R group, RPS (10, 20, 40μg/ml)treatment groups and SXN (100μg/ml)treatment group (n=10). 6 hours after the drugs were given, the morphology changes was observed and the survival rate was determined by MTT; the activity of AST, CK, LDH in culture medium were detected; the activity of SOD, CAT, GSH-Px and the content of MDA in cardiomyocytes were also determinted; the apoptosis rate were detected. Results Compared with the H/R group, the morphology of cardiomyocytes in RPS treatment groups were improved, the survival rate was significantly increased, the activity of CPK, AST and LDH in culture medium were significantly decreased, the activity of SOD, GSH-Px, CAT in cardiomyocytes were significantly increased and the content of MDA was significantly decreased, the apoptosis rate was significantly decreased. All of the difference in RPS 40μg/ml treatment group was the most significant (P<0.05, P<0.01). Conclusion RPS could effectively improve the morphology of cardiomyocytes impaired by H/R, increase the survival rate, improve the activity of antioxidase, depress oxidative stress and decrease the apoptosis rate, suggesting that RPS had protective effects on cadiocytes of neonatal rat impaired by H/R. |
| keywords:RPS Neonatal rat Cardiomyocytes Hypoxia-reoxygenation Protection |
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