| 苯并芘通过NF-κB诱导RAW264.7细胞MMPs的表达 |
| 投稿时间：2015-10-23 修订日期：2015-11-05 点此下载全文 |
| 引用本文：王国强,郭辉辉,吴少泽,陶路渊,薛杨静,唐疾飞,季亢挺.苯并芘通过NF-κB诱导RAW264.7细胞MMPs的表达[J].医学研究杂志,2016,45(5):63-66 |
| DOI：
10.11969/j.issn.1673-548X.2016.05.015 |
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| 基金项目:国家自然科学基金资助项目(81573185);浙江省科技厅基金资助项目(2014C33163);温州科技局计划项目(Y20140678) |
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| 中文摘要:目的 探讨烟雾主要成分苯并芘(BaP)对RAW264.7单核-吞噬细胞中基质金属蛋白酶(MMPs)的影响,为研究吸烟与腹主动脉瘤的关联提供相关依据。方法 应用免疫印迹法观察BaP对体外培养的RAW264.7单核-吞噬细胞基质金属蛋白酶-2/9/12(MMP-2/9/12)的影响,并验证其是否通过NF-κB信号通路。结果 BaP暴露使培养巨噬细胞NF-κB活化,同时伴随MMP-9、MMP-12表达升高,而PDTC,核转录因子NF-κB的抑制剂,可抑制NF-κB活化,同时伴随MMP-9、MMP-12表达下降。结论 BaP可通过NF-κB通路诱导RAW264.7单核-吞噬细胞MMP-9、MMP-12表达升高。 |
| 中文关键词:单核-吞噬细胞 苯并芘 核转录因子κB 基质金属蛋白酶 |
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| Benzo[a]pyrene (BaP) Induced the Expression of MMPs in the RAW2645 Cell through the NF-κB |
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| Abstract:Objective To explore the effect of benzopyrene(BaP),the maincomponents of smoke,on matrix metalloproteinase(MMPs) of RAW246.7 mononuclear macrophage. Methods We observed the influence of BaP on matrix metalloproteinase-2/9/12(MMP-2/9/12) of RAW246.7 mononuclear macrophage in vitro by western blotting and verified if it happened through the NF-κB signaling pathway. Results BaP caused NF-κB activation and expression of MMP-12, MMP-9 increased in cultured macrophages.While PDTC,an inhibitor of nuclear transcription factor NF-κB,caused the inhibition of NF-κB activation and expression of MMP-12 and MMP-9 decreased. Conclusion BaP can induce the expression of MMP-9 and MMP-12 in RAW246.7 cell by NF-κB pathway. |
| keywords:Monocyte macrophages Benzopyrene Nuclear transcription factor kappa B Matrix metalloproteinases |
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