小檗碱通过Notch途径抑制人T淋巴细胞性白血病Molt-4细胞增殖及凋亡的影响
投稿时间:2015-11-03  修订日期:2015-12-01  点此下载全文
引用本文:王冠玲,胡斌飞.小檗碱通过Notch途径抑制人T淋巴细胞性白血病Molt-4细胞增殖及凋亡的影响[J].医学研究杂志,2016,45(6):167-171
DOI: 10.11969/j.issn.1673-548X.2016.06.043
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作者单位E-mail
王冠玲 315012 宁波市妇女儿童医院 wanggldoc@163.com 
胡斌飞 315012 宁波市妇女儿童医院  
中文摘要:目的 探讨小檗碱对人T淋巴细胞白血病细胞株Molt-4细胞体外增殖抑制、凋亡诱导及相关机制。方法 CCK-8法检测不同浓度的小檗碱对Molt-4细胞的增殖抑制作用;流式细胞术检测细胞凋亡率;RT-PCR检测Molt-4细胞Notch1、Bcl-xl、Deltex1基因表达情况。结果 小檗碱诱导Molt-4细胞毒性及凋亡作用,呈剂量依赖性(P<0.05);RT-PCR显示Molt-4细胞株有Notch1 mRNA的表达,且随着小檗碱剂量的增加,Notch1、Bcl-xl、Deltex1 mRNA的表达逐渐下降。结论 小檗碱可能通过Notch信号途径抑制Molt-4细胞增殖并诱导凋亡。
中文关键词:小檗碱  Molt-4细胞  Notch1信号途径  凋亡
 
Effects of Berberine on the Proliferation and Apoptosis in Molt-4 cell through Notch Signaling Pathway
Abstract:Objective To investigate the effects of berberine on the proliferation,apoptosis and explore its mechanism in human T lymphoblastic leukemia cell line (Molt-4). Methods Molt-4 cells were treated with different concentrations of berberine in vitro respectively. Antiproliferative effect was detected by CCK-8 assay.Apoptosis rate was observed by flow cytometry (FCM).The expression of Notch1, Bcl-xl and Deltex1 mRNA was examined by Semi-quantitative reverse transcription PCR (RT-PCR). Results Berberine induced cytotoxicity and apoptosis in Molt-4 cells in a dose-dependent manner (P<0.05).RT-PCR results showed that the expression of Notch1, Bcl-xl and Deltex1 genes decreased with the increase of berberine concentration (P<0.05). Conclusion Berberine could inhibit the proliferation of Molt-4 cells and promote the apoptosis through down-regulation expression of apoptotic protein mediated by Notch signaling pathway.
keywords:Berberine  Molt-4 cells  Notch1 cell signaling pathway  Cell apoptosis
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