| 小檗碱通过Notch途径抑制人T淋巴细胞性白血病Molt-4细胞增殖及凋亡的影响 |
| 投稿时间：2015-11-03 修订日期：2015-12-01 点此下载全文 |
| 引用本文：王冠玲,胡斌飞.小檗碱通过Notch途径抑制人T淋巴细胞性白血病Molt-4细胞增殖及凋亡的影响[J].医学研究杂志,2016,45(6):167-171 |
| DOI：
10.11969/j.issn.1673-548X.2016.06.043 |
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| 中文摘要:目的 探讨小檗碱对人T淋巴细胞白血病细胞株Molt-4细胞体外增殖抑制、凋亡诱导及相关机制。方法 CCK-8法检测不同浓度的小檗碱对Molt-4细胞的增殖抑制作用；流式细胞术检测细胞凋亡率；RT-PCR检测Molt-4细胞Notch1、Bcl-xl、Deltex1基因表达情况。结果 小檗碱诱导Molt-4细胞毒性及凋亡作用，呈剂量依赖性（P<0.05）；RT-PCR显示Molt-4细胞株有Notch1 mRNA的表达，且随着小檗碱剂量的增加，Notch1、Bcl-xl、Deltex1 mRNA的表达逐渐下降。结论 小檗碱可能通过Notch信号途径抑制Molt-4细胞增殖并诱导凋亡。 |
| 中文关键词:小檗碱 Molt-4细胞 Notch1信号途径 凋亡 |
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| Effects of Berberine on the Proliferation and Apoptosis in Molt-4 cell through Notch Signaling Pathway |
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| Abstract:Objective To investigate the effects of berberine on the proliferation,apoptosis and explore its mechanism in human T lymphoblastic leukemia cell line (Molt-4). Methods Molt-4 cells were treated with different concentrations of berberine in vitro respectively. Antiproliferative effect was detected by CCK-8 assay.Apoptosis rate was observed by flow cytometry (FCM).The expression of Notch1, Bcl-xl and Deltex1 mRNA was examined by Semi-quantitative reverse transcription PCR (RT-PCR). Results Berberine induced cytotoxicity and apoptosis in Molt-4 cells in a dose-dependent manner (P<0.05).RT-PCR results showed that the expression of Notch1, Bcl-xl and Deltex1 genes decreased with the increase of berberine concentration (P<0.05). Conclusion Berberine could inhibit the proliferation of Molt-4 cells and promote the apoptosis through down-regulation expression of apoptotic protein mediated by Notch signaling pathway. |
| keywords:Berberine Molt-4 cells Notch1 cell signaling pathway Cell apoptosis |
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