肾小管上皮细胞在高糖环境下其间隙连接蛋白与细胞通透性的关系
投稿时间:2015-11-22  修订日期:2015-12-22  点此下载全文
引用本文:杨黎,哈伟杰,王真.肾小管上皮细胞在高糖环境下其间隙连接蛋白与细胞通透性的关系[J].医学研究杂志,2016,45(6):183-186
DOI: 10.11969/j.issn.1673-548X.2016.06.047
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作者单位E-mail
杨黎 100091 北京世纪坛医院急诊科  
哈伟杰 100091 北京世纪坛医院急诊科  
王真 102600 北京市大兴区人民医院急诊科 wangzhen1369@hotmail.com 
中文摘要:目的 观察肾小管上皮细胞在不同浓度葡萄糖干预下其间隙连接蛋白(connexin43)表达与细胞通透性的关系。方法 用不同浓度葡萄糖刺激肾小管上皮细胞,通过免疫荧光法检测connexin43蛋白的表达;用RT-PCR分析connexin43 mRNA的表达。使用Transwell小室法测定各组细胞对白蛋白的通透情况,并观察以上指标之间的变化有无相关性。结果 肾小管上皮细胞在高糖干预下间隙连接蛋白表达递减,单层肾小管上皮细胞通透性升高。结论 高糖环境下肾小管上皮细胞间隙连接蛋白的表达与细胞对白蛋白的通透性呈负相关。
中文关键词:间隙连接蛋白  上皮细胞  肾小管  白蛋白  通透性
 
Relationship of Connexin43 Expression and the Cellular Permeability in High Glucose Stimulated HKC
Abstract:Objective To observe the expression of gap junction protein (connexin43) and the permeability of albumin of cultured human kidney tubular epithelial cells(HKC) which was stimulated with glucose,and the relationship between the expression of connexin 43 and the permeability of HKC. Methods The normal HKC was stimulated with glucose to detecte the expression of connexin43 protein of HKC in different groups. The expression of connexin43 protein and mRNA was detected by Immunity fluorescence and RT-PCR.Using Transwell cab we examined the permeability of albumin in different groups, and observed whether there was relevance between the connexin43 expression and the cellular permeability. Results Different concentrations of glucose stimulated HKC,down-regulated the connexin43 protein expression,and up-regulated the cell permeability. Conclusion The expression of gap junction protein and the permeability of HKC in high glucose is relevant.
keywords:Connexin43  Epithelial cell  Kidney tubule  Albumin  Permeability
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