| 自体干细胞激活疗法治疗2型糖尿病大鼠的疗效及安全性探讨 |
| 投稿时间：2013-07-01 修订日期：2016-03-09 点此下载全文 |
| 引用本文：郭新红,郑滨珠,袁凤山.自体干细胞激活疗法治疗2型糖尿病大鼠的疗效及安全性探讨[J].医学研究杂志,2016,45(10):64-67 |
| DOI：
10.11969/j.issn.1673-548X.2016.10.016 |
| 摘要点击次数: 867 |
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| 基金项目:辽宁省科技厅科学技术计划项目（2012225014） |
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| 中文摘要:目的 本实验采用自体干细胞激活疗法治疗糖尿病大鼠过程中对血细胞及生化指标进行了监测，以观察糖尿病干细胞治疗的效果及治疗过程中可能存在的不良反应。方法 46只大鼠随机分为健康对照组10只，拟造模组大鼠36只。予高糖高脂加小剂量链脲佐菌素（30mg/kg）制备糖尿病大鼠模型。造模成功后30只大鼠随机分为糖尿病对照组10只、干细胞治疗组 20只。干细胞治疗组予重组人粒细胞集落刺激因子[3μg/（kg·d）]隔日1次皮下注射，肝细胞生长因子[3mg/（kg·d）]腹腔注射及烟酰胺[100mg/（kg·d）]灌胃；两对照组大鼠予同体积生理盐水隔日1次皮下注射，每日同体积5%葡萄糖加中和量胰岛素腹腔注射及高压灭菌水灌胃。实验过程中，各组大鼠测定血糖、血常规及生化指标。结果 治疗后1周开始干细胞治疗组较糖尿病对照组血糖显著下降（P<0.01）；治疗1~4周时干细胞治疗组较两对照组WBC、PLT明显升高（P<0.05）；治疗前后3组大鼠ALT、GGT无显著变化。治疗前糖尿病对照组较健康对照组大鼠ALP显著升高（P<0.05），治疗后干细胞治疗组较两对照组大鼠ALP升高更为显著。结论 2型糖尿病大鼠采用自体干细胞激活疗法治疗后，其血糖明显下降，证实糖尿病干细胞治疗有效；WBC、PLT及ALP升高，表明干细胞被成功动员；RBC、LYM计数及ALT及GGT浓度无明显变化；所有改变未见相关不良反应。 |
| 中文关键词:糖尿病 骨髓干细胞 烟酰胺 肝细胞生长因子 碱性磷酸酶 |
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| Curative Effect and Safety of Autologous Stem Cell Activation Therapy to Treat Type 2 Diabetic Rats |
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| Abstract:Objective To observe the effect and possible side effects of the stem cells to treat diabetes by monitoring the changes of blood and biochemical index. Methods Forty xis rats were randomly divided into 10 healthy rats of control group, 36 rats of model group. Chain of high-sugar and high-fat diet plus small dose of streptozotocin (30mg/kg) were giren to build the diabetic rats model. The successful model rats were randomly divided into diabetic control group with 10 rats and stem cell treatment group with 20 rats. Rats the two control group were given daily intraperitoneal injection of saline water and high pressure sterilization lavage, 5% glucose plus quantity insulin with a subcutaneous injection.stem. Rats of the stem cell treatment group were given recombinant human granulcyte-colony stimulating factor(3mg/kg)by subcutaneous injection at a time, daily hepatocyte growth factor (23mg/kg) by intraperitoneal injection and niacinamide (100mg/kg) filled the stomach. During tratment process, the blood sugar, blood routine and biochemical indexes in rats were determind. Results After 1 week treatment, compared with the diabetic control group, the blood glucose of the stem cell treatment group rats decreased significantly (P<0.01).Before treatment, the RBC, WBC, LYM and PLT of the three group rats had no obvious differences. At 1 week and 4 week after treatment, compared with the two controls the WBC and PLT of the group rats increased significantly (P<0.05). Before and after treatment, the ALT and GGT of the three groups had no significant changes. Before treatment, ALP was higher in the diabetes control group rats than the healthy control group (P<0.05), and after treatment, compared with two controls, the ALP of the stem cell treatment group rats increased more significantly. Conclusion The blood sugar of the diabetic rats treated by autologous stem cell activation therapy drops which confirms the effect of the stem cell treatment for diabetes. The increased of WBC, PLT and ALP suggestes that the stem cells were successfully mobilized, RBC, LYM and ALT, GGT had no obvious changes. All changes have no related adverse reactions in the treatment. |
| keywords:Diabetes mellitus Bone marrow stem cells Nicotinamide hepatocyte growth factor Alkaline phosphatase |
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