| 牙龈间充质干细胞在大鼠体内分布情况及其分离鉴定 |
| 投稿时间：2016-05-10 修订日期：2016-06-07 点此下载全文 |
| 引用本文：竺王玉,朱蓓,陈冬冬.牙龈间充质干细胞在大鼠体内分布情况及其分离鉴定[J].医学研究杂志,2017,46(1):97-100,11 |
| DOI：
10.11969/j.issn.1673-548X.2017.01.027 |
| 摘要点击次数: 942 |
| 全文下载次数: 667 |
|
| 基金项目:浙江省科技厅公益类项目（2014C33244） |
|
| 中文摘要:目的 了解一种新的牙龈来源的间充质干细胞（gingival tissue-derived mesenchymal stem cells，GMSCs）在大鼠体内的分布情况及其分离鉴定。方法 取成人健康牙龈组织，经dispaseⅡ和胶原酶Ⅳ消化分离单细胞，培养并鉴定为MSCs。将36只SD大鼠（雌雄各半）分成两组：GMSCs移植组（n=24）和对照组（n=12）。GMSCs经尾静脉输入，并分别于移植后1、5、10、20和30天各时间点随机处死4只大鼠，快速取出心脏、肝脏、脾脏、肺脏、肾脏、脑、胸骨、肌肉、睾丸或卵巢等组织，冷冻切片后荧光显微镜观察GMSCs生物分布。结果 GMSCs贴壁生长，呈长梭形，具有向脂肪细胞及成骨细胞分化的能力，CD29、CD39、CD73、CD90、CD105阳性率>95%，CD34、CD45、HLA-DR阳性率<5%。荧光显微镜观察结果显示，移植后1天肺CM-Dil阳性细胞数最高，其次为肝、淋巴结、胸腺、脾、肾、骨髓、外周血、卵巢。移植后肺、肝CM-Dil阳性细胞数缓慢下降；移植后10天淋巴结和胸腺CM-Dil阳性细胞数下降明显，但以后保持稳定。GMSCs移植大鼠后1~30天，在心、脑、睾丸、附睾、子宫、肌肉、皮肤等均未见有CM-Dil阳性细胞。结论 GMSCs移植大鼠后，在肺、肝、肾及免疫器官淋巴结、脾、胸腺分布较多，GMSCs移植后具有其特殊的生物分布特征，对将来指导GMSCs用于免疫治疗具有重要意义。 |
| 中文关键词:间充质干细胞 牙龈 分布 CM-Dil |
| |
| Isolation and Identification for Gingival Derived Mesenchymal Stem Cells and Its Distribution in SD Mice |
|
|
| Abstract:Objective To study the isolation and identification for a new gingival tissue derived mesenchymal stem cells (GMSCs) and its distribution in SD mice. Methods Healthy gingival tissue was obtained from adults, and then was digested by dispaseⅡand collagen for separating single cell which were cultured later and identified as MSCs. Thirty six SD mice were divided into two groups (gender ratio=1:1):GMSCs transplantation group (n=24) and the control group (n=12). GMSCs transplanted to SD mice via tail vein injection, and sacrificed 4 mice at day 1, day 5, day 10, day 20 and day 30 after transplantation, respectively. Mice's heart, liver, spleen, lung, kidney, brain, bone, muscle, testis or ovary tissue were taken out, frozen sliced and then observed by fluorescent microscope to determine GMSCs biological distribution. Results GMSCs were adherent growth, like a long fusiform and had the ability to differentiate to adipocytes or osteoblasts. The positive rate of CD29, CD39, CD73, CD90, CD105 were more than 95%, whereas the positive rate of CD34, CD45, HLA-DR were less than 5%. The observation of fluorescence microscopy showed CM-Dil positive cells were highest in lung after 1 day transplantation, followed by liver, lymph nodes, thymus, spleen, kidney, bone marrow, peripheral blood and ovaries. The CM-Dil positive cells were slow down in lung and liver after transplantation; while they were decreased obviously in lymph node and thymus after 10 day transplantation and then remained stable. However, no CM-Dil positive cell was found in heart, brain, testis, epididymis, uterus, muscle and skin after GMSCs transplantation. Conclusion Lung, liver, kidney and immune organ such as lymph node, spleen and thymus distributed more GMSCs than other organs after transplantation to the mice. GMSC has its special biological distributed characteristics, which is important for the guidance of GMSCs immunotherapy in near future. |
| keywords:Mesenchymal stem cells Gingival Distribution CM-Dil |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
