| 特发性血小板减少性紫癜患儿细胞免疫功能变化及临床意义 |
| 投稿时间：2016-05-05 修订日期：2016-06-21 点此下载全文 |
| 引用本文：阮毅燕,陈瑜毅,唐文珏,胡雪桦,陆盅妤,冯军坛,谢翠玲,刘敏成.特发性血小板减少性紫癜患儿细胞免疫功能变化及临床意义[J].医学研究杂志,2017,46(2):43-45 |
| DOI：
10.11969/j.issn.1673-548X.2017.02.013 |
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| 基金项目:中国疾病预防控制中心妇幼保健中心科研项目（2013FY015） |
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| 中文摘要:目的 探讨特发性血小板减少性紫癜（ITP）患儿细胞免疫功能的变化及其临床意义。方法 将2013年8月~2015年7月在笔者医院儿科住院的83例ITP初治患儿及31例同期在笔者医院门诊体检的健康同龄儿纳入研究，采用流式细胞术检测外周血T淋巴细胞亚群CD3、CD4、CD8、CD4/CD8比例，采用酶联免疫吸附试验检测血浆细胞因子IFN-γ及IL-4浓度。结果 治疗前病例组CD3 60.07%±11.85%、CD4 34.18%±11.72%、CD4/CD8 1.76%±0.94%及IL-4 11.24±8.42pg/ml水平显著低于对照组70.96%±7.26%、45.05%±6.53%，2.26%±0.66%及21.26±9.34pg/ml，IFN-γ水平5.88±4.02pg/ml显著高于对照组2.12±1.09pg/ml；治疗后8周后，CD3 65.76%±10.87%、CD4 38.71%±11.81%及IL-4 14.59±11.59pg/ml水平均较治疗前升高，IFN-γ 3.65±3.15pg/ml水平均较治疗前降低，差异有统计学意义（P < 0.01或P < 0.05）。结论 ITP患儿存在细胞免疫功能紊乱，Thl/Th2平衡失调可能与ITP的发病机制和疾病的活动状态密切相关。 |
| 中文关键词:血小板减少性紫癜 儿童 细胞免疫 淋巴细胞亚群 细胞因子 |
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| Changes of Cellular Immune Function in Children with Idiopathic Thrombocytopenic Purpura and Its Clinical Significance |
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| Abstract:Objective To investigation the changes of cellular immune function in children with idiopathic thrombocytopenic purpura(ITP) and its clinical significance. Methods Eighty-three children with ITP hospitalized in our hospital from August 2013 to July 2015 and 31 healthy children as control group were enrolled in this study. The proportion of T lymphocyte CD3, CD4, CD8, CD4/CD8 in peripheral blood of the patients and healthy subsets were analyzed by flow cytometry. The plasma concentrations of cytokines IFN-γ and IL-4 were detected by ELISA. Results ① Compared with the control group, the levels of CD3 60.07%±11.85%,CD4 34.18%±11.72%,CD4/CD8 1.76%±0.94% and IL-4 11.24±8.42pg/ml were significantly lower and IFN-γ 5.88±4.02pg/ml was significantly higher in the case group before treatment. ② After 8 weeks of treatment, the levels of CD3 65.76%±10.87%, CD4 38.71%±11.81% and IL-4 14.59±11.59pg/ml were significantly increased and IFN-γ 3.65±3.15pg/ml was significantly decreased than before(P < 0.01 or P < 0.05). Conclusion There is a disorder of cellular immune function in children with ITP, and the imbalance of Thl/Th2 may be closely related to the pathogenesis of ITP and the active state of the disease. |
| keywords:Idiopathic thrombocytopenic purpura Child Cellular immune Lymphocyte Cytokine |
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