| 大蒜素对大鼠肾缺血再灌注氧化应激损伤保护作用的研究 |
| 投稿时间：2016-05-31 修订日期：2016-06-30 点此下载全文 |
| 引用本文：杨杰,胡亚丽,郑茂东,梁江.大蒜素对大鼠肾缺血再灌注氧化应激损伤保护作用的研究[J].医学研究杂志,2017,46(2):108-111 |
| DOI：
10.11969/j.issn.1673-548X.2017.02.028 |
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| 中文摘要:目的 研究大蒜素对大鼠肾缺血再灌注后氧化应激损伤的保护作用。方法 通过夹闭双侧肾蒂血管45min的方法建立肾缺血再灌注损伤大鼠模型，造模前7天分别给予不同剂量大蒜素（5、10、20mg/kg）和银杏叶提取物（100mg/kg）进行预处理，同时设假手术组和模型组；再灌注6h后计算肾脏指数，生化分析法测定肾功能指标（BUN、SCr、UA），苏木精-伊红（HE）染色法观察肾脏组织形态结构改变，TUNEL染色法观察细胞凋亡状况；RT-PCR法测定Bax mRNA、bcl-2 mRNA表达；测定肾脏组织中抗氧化酶（SOD、GSH-Px、CAT）活性和丙二醛（MDA）含量；Western blot法检测NF-κB蛋白表达。结果 经大蒜素预处理能够显降低肾缺血再灌注损伤大鼠肾脏指数，降低血清中BUN、SCr、UA含量，改善肾脏组织病变和细胞凋亡状况，下调肾脏组织中Bax mRNA表达且上调bcl-2mRNA表达、显著降低Bax/bcl-2比值，提高抗氧化酶（SOD、GSH-Px、CAT）活性并显著降低MDA含量，显著下调NF-κB蛋白表达，上述作用均具有一定的剂量依赖性。结论 大蒜素可能通过抑制氧化应激损伤而对大鼠肾缺血再灌注大鼠具有一定的保护作用。 |
| 中文关键词:大蒜素 肾脏 缺血再灌注 氧化应激 保护 |
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| Protective Effect of Allitridi on Oxidative Stress in the Rats with Renal Ischemic-Reperfusion Injury |
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| Abstract:Objective To investigate the protective effect of Allitridi on oxidative stress in the rats with renal ischemic-reperfusion injury. Methods The model rats were made by gripping bilateral renal pedicle vascular for 45min; and the experimental rats were pre-treated with Allitridi(5, 10, 20mg/kg) groups and ginkgo biloba extract(EGb) 100mg/kg. Six hours after reperfusion, the renal index(RI) was calculated, the content of BUN, SCr, UA in serum were determined, the renal tissue histopathological changes was observed by HE staining, the apoptosis of renal cells was observed by TUNEL staining, the expression level of Bax, bcl-2 mRNA were detected by RT-PCR, the activity of SOD, GSH-Px, CAT and the content of MDA in renal tissue were determined, the expression of NF-κB in renal tissue was detected by Western blot. Results The RI in Allitridi pre-treated groups were significantly decreased. The contentes of BUN, SCr, UA were significantly decreased. The histopathological changes and the apoptosis in puerarin treated groups were significantly improved, especially the Allitridi 40mg/kg treated group. The AI in Allitridi pre-treated groups were significantly decreased. The expression levels of bax mRNA in Allitridi pre-treated groups were significantly decreased and the bcl-2 mRNA was significantly increased, and the bax/bcl-2 was significantly decreased. The activity of SOD, GSH-Px, CAT in renal tissue of Allitridi pre-treated groups were significantly increased and the contentes of MDA were significantly decreased. And the expression of NF-κB was significantly decreased, which were dose-dependent. Conclusion Allitridi has protective effects on the rats with renal ischemic-reperfusion injury perhaps through depressing oxidative stess. |
| keywords:Allitridi Renal tissue Ischemic-reperfusion Oxidative stress Protection |
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