| 鸡骨草冷浸提取物抗病毒活性研究 |
| 投稿时间：2016-11-02 修订日期：2016-11-11 点此下载全文 |
| 引用本文：刘相文,侯林,张成华,李雅群,刘金安,田景振.鸡骨草冷浸提取物抗病毒活性研究[J].医学研究杂志,2017,46(6):60-62 |
| DOI：
10.11969/j.issn.1673-548X.2017.06.016 |
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| 基金项目:国家重大新药创制专项基金资助项目（2014ZX09509001）；山东省高等学校中医药抗病毒协同创新中心基金资助项目（XTCX2014C01-01） |
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| 中文摘要:目的 研究中药鸡骨草冷浸提取物的抗病毒活性。方法 采用细胞体外抗病毒实验法，结合CPE法及CCK-8试剂盒，以TI值（治疗指数）为研究指标，以利巴韦林、阿昔洛韦为阳性对照药，研究鸡骨草冷浸提取物对呼吸道合胞病毒（RSV）、单纯疱疹病毒（HSV-1）、柯萨奇病毒（COX-B5）和手足口病毒（EV71）的抑制作用。结果 甲醇冷浸提取物抗病毒效果较好，对COX-B5的TI值为16.476，最大抑制率为88%，与阳性对照药利巴韦林（TI值为17.482，最大抑制率为85%）相差不大。结论 鸡骨草冷浸提取物具有体外抗RSV、HSV-1、COX-B5的活性。 |
| 中文关键词:鸡骨草 抗病毒 RSV HSV-1 COX-B5 |
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| Antiviral Activity of Cold-soak Extraction of Abrus Cantoniensis Hance |
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| Abstract:Objective To investigate the antiviral activity of cold-soak extraction of Abrus cantoniensis Hance. Methods Four different strains of viruses, including RSV, HSV-1,COX-B5 and EV71 were used for in-vitro antivirus experiment, and the antiviral activity of Abrus cantoniensis Hance were evaluated by the cytopathic effect (CPE) method and CCK-8.Thepapeutic indexes were calculated by the Reed-Muench method. Antivirus stability experiments of Abrus cantoniensis Hance were carried out at the same time. Results The TI of Methanol cold-soak extraction and Ribavirin were 16.476 and 17.482. It showed Methanol cold-soak extraction was as effective as the Ribavirin. Conclution It indicated that cold-soak extraction of Abrus cantoniensis Hance have antiviral activity against RSV, HSV-1 and COX-B5. |
| keywords:Abrus cantoniensis Hance Antiviral RSV HSV-1 COX-B5 |
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