| miR-15b在恶性黑色素瘤A375细胞中的表达及其对细胞转移和侵袭的影响 |
| 投稿时间：2016-11-01 修订日期：2016-11-28 点此下载全文 |
| 引用本文：金丹,周烈,刘启祥,吴明勇,全柳霞,叶夏潮,刘旭阳,肖修兰.miR-15b在恶性黑色素瘤A375细胞中的表达及其对细胞转移和侵袭的影响[J].医学研究杂志,2017,46(7):156-159 |
| DOI：
10.11969/j.issn.1673-548X.2017.07.039 |
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| 中文摘要:目的 研究微小RNA-15b（microRNA-15b，miR-15b）在恶性黑色素瘤A375细胞中的表达，以及miR-15b对恶性黑色素瘤细胞转移和侵袭能力的影响。方法 应用荧光实时定量聚合酶链反应（quantitative real-time polymerase chain reaction， qRT-PCR）方法检测恶性黑色素瘤A375细胞与正常黑素细胞HM中miR-15b的表达水平。沉默恶性黑色素瘤A375细胞中miR-15b的表达后，应用划痕实验检测miR-15b对恶性黑色素瘤A375细胞迁移距离的影响，应用Transwell实验检测miR-15b对恶性黑色素瘤A375细胞转移和侵袭能力的影响。结果 miR-15b恶性黑色素瘤A375细胞中的表达水平显著高于正常黑素细胞。沉默恶性黑色素瘤细胞中miR-15b的表达后，细胞迁移距离、细胞转移和侵袭能力均受到显著抑制。结论 miR-15b在恶性黑色素瘤A375细胞中表达显著升高并可促进恶性黑色素瘤A375细胞转移和侵袭。 |
| 中文关键词:miR-15b 黑色素瘤 转移 侵袭 |
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| Expression of MIR-15B in Malignant Melanoma and Its Effects on Cell Migration and Invasion |
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| Abstract:Objective To investigate the expression of miR-15b in malignant melanoma cell line A375,and further investigate the effects of miR-15b on cell migration and invasion of A375 cells. Methods The expression of miR-15b in A375 cells and human melanocyte cell lines HM was detected by qRT-PCR. After silencing the expression of miR-15b in A375 cells, we detected the effects of miR-15b on cell migrating distance by wound healing assays, we also detected the effects of miR-15b on cell migration and invasion ability by Transwell assays. Results The expression of miR-15b was significantly upreglated in A375 cells than HM cells. After silencing the expression of miR-15b in A375 cells, the cell migrating distance, cell migration and cell invasion was significantly inhibited. Conclusion miR-15B was upregulated in malignant melanoma cell line A375, and could promote the migration and invasion of A375 cells. |
| keywords:miR-15b Melanoma Migration Invasion |
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